{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["White LK"],"funding":["RNA Bioscience Initiative","the Molecular Biology Program, the Bolie Family Foundation","National Institutes of Health","NIGMS NIH HHS"],"pagination":["847-861"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10187680"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["29(6)"],"pubmed_abstract":["Ligation by plant and fungal RNA ligases yields an internal 2'-phosphate group on each RNA ligation product. In budding yeast, this covalent mark occurs at the splice junction of two targets of ligation: intron-containing tRNAs and the messenger RNA <i>HAC1</i> The repertoire of RNA molecules repaired by RNA ligation has not been explored due to a lack of unbiased approaches for identifying RNA ligation products. Here, we define several unique signals produced by 2'-phosphorylated RNAs during nanopore sequencing. A 2'-phosphate at the splice junction of <i>HAC1</i> mRNA inhibits 5' → 3' degradation, enabling detection of decay intermediates in yeast RNA repair mutants by nanopore sequencing. During direct RNA sequencing, intact 2'-phosphorylated RNAs on <i>HAC1</i> and tRNAs produce diagno"],"journal":["RNA (New York, N.Y.)"],"pubmed_title":["Nanopore sequencing of internal 2'-PO<sub>4</sub> modifications installed by RNA repair."],"pmcid":["PMC10187680"],"funding_grant_id":["T32 GM136444","R35 GM119550"],"pubmed_authors":["Strugar SM","White LK","MacFadden A","Hesselberth JR"],"additional_accession":[]},"is_claimable":false,"name":"Nanopore sequencing of internal 2'-PO<sub>4</sub> modifications installed by RNA repair.","description":"Ligation by plant and fungal RNA ligases yields an internal 2'-phosphate group on each RNA ligation product. In budding yeast, this covalent mark occurs at the splice junction of two targets of ligation: intron-containing tRNAs and the messenger RNA <i>HAC1</i> The repertoire of RNA molecules repaired by RNA ligation has not been explored due to a lack of unbiased approaches for identifying RNA ligation products. Here, we define several unique signals produced by 2'-phosphorylated RNAs during nanopore sequencing. A 2'-phosphate at the splice junction of <i>HAC1</i> mRNA inhibits 5' → 3' degradation, enabling detection of decay intermediates in yeast RNA repair mutants by nanopore sequencing. During direct RNA sequencing, intact 2'-phosphorylated RNAs on <i>HAC1</i> and tRNAs produce diagno","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2026-07-15T20:04:04.692Z","creation":"2026-07-09T03:10:14.148Z"},"accession":"S-EPMC10187680","cross_references":{"pubmed":["36854608"],"doi":["10.1261/rna.079290.122"]}}