{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["41(5)"],"submitter":["Lam DK"],"pubmed_abstract":["Cytosine base editors (CBEs) enable programmable genomic C·G-to-T·A transition mutations and typically comprise a modified CRISPR-Cas enzyme, a naturally occurring cytidine deaminase, and an inhibitor of uracil repair. Previous studies have shown that CBEs utilizing naturally occurring cytidine deaminases may cause unguided, genome-wide cytosine deamination. While improved CBEs that decrease stochastic genome-wide off-targets have subsequently been reported, these editors can suffer from suboptimal on-target performance. Here, we report the generation and characterization of CBEs that use engineered variants of TadA (CBE-T) that enable high on-target C·G to T·A across a sequence-diverse set of genomic loci, demonstrate robust activity in primary cells and cause no detectable elevation in g"],"journal":["Nature biotechnology"],"pagination":["686-697"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10188367"],"repository":["biostudies-literature"],"pubmed_title":["Improved cytosine base editors generated from TadA variants."],"pmcid":["PMC10188367"],"pubmed_authors":["Lee KD","Peraro L","Lee SJ","Feliciano PR","Sawyer C","Arif A","Bohnuud T","Ortega MA","Gaudelli NM","Gehrke JM","Schwaegerle ND","Lam DK","Ciaramella G","Young L","Fernandez TP","Grayson P"],"additional_accession":[]},"is_claimable":false,"name":"Improved cytosine base editors generated from TadA variants.","description":"Cytosine base editors (CBEs) enable programmable genomic C·G-to-T·A transition mutations and typically comprise a modified CRISPR-Cas enzyme, a naturally occurring cytidine deaminase, and an inhibitor of uracil repair. Previous studies have shown that CBEs utilizing naturally occurring cytidine deaminases may cause unguided, genome-wide cytosine deamination. While improved CBEs that decrease stochastic genome-wide off-targets have subsequently been reported, these editors can suffer from suboptimal on-target performance. Here, we report the generation and characterization of CBEs that use engineered variants of TadA (CBE-T) that enable high on-target C·G to T·A across a sequence-diverse set of genomic loci, demonstrate robust activity in primary cells and cause no detectable elevation in g","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-05-28T08:26:14.862Z","creation":"2025-04-05T21:10:01.028Z"},"accession":"S-EPMC10188367","cross_references":{"pubmed":["36624149"],"doi":["10.1038/s41587-022-01611-9"]}}