{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["3(5)"],"submitter":["Li W"],"pubmed_abstract":["This study was conducted to optimize a targeted plant proteomics approach from signature peptide selection and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analytical method development and optimization to sample preparation method optimization. Three typical protein extraction and precipitation methods, including trichloroacetic acid (TCA)/acetone method, phenol method, and TCA/acetone/phenol method, and two digestion methods, including trypsin digestion and LysC/trypsin digestion, were evaluated for selected proteins related to the impact of engineered nanomaterials (ENMs) on wheat (<i>Triticum aestivum</i>) plant growth. In addition, we compared two plant tissue homogenization methods: grinding freeze-dried tissue and fresh tissue into a fine powder using a mortar and "],"journal":["ACS agricultural science & technology"],"pagination":["421-431"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10189723"],"repository":["biostudies-literature"],"pubmed_title":["Optimization of Targeted Plant Proteomics Using Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)."],"pmcid":["PMC10189723"],"pubmed_authors":["Li W","Keller AA"],"additional_accession":[]},"is_claimable":false,"name":"Optimization of Targeted Plant Proteomics Using Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS).","description":"This study was conducted to optimize a targeted plant proteomics approach from signature peptide selection and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analytical method development and optimization to sample preparation method optimization. Three typical protein extraction and precipitation methods, including trichloroacetic acid (TCA)/acetone method, phenol method, and TCA/acetone/phenol method, and two digestion methods, including trypsin digestion and LysC/trypsin digestion, were evaluated for selected proteins related to the impact of engineered nanomaterials (ENMs) on wheat (<i>Triticum aestivum</i>) plant growth. In addition, we compared two plant tissue homogenization methods: grinding freeze-dried tissue and fresh tissue into a fine powder using a mortar and ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-21T22:44:07.058Z","creation":"2025-04-21T22:44:07.058Z"},"accession":"S-EPMC10189723","cross_references":{"pubmed":["37206883"],"doi":["10.1021/acsagscitech.3c00017"]}}