{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Qin Y"],"funding":["Guangdong Province Science and Technology Project","Guangzhou City Science and Technology","Natural Science Foundation of Guangdong Province"],"pagination":["40"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10189940"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(1)"],"pubmed_abstract":["<h4>Background</h4>Carbapenemase-producing makes a great contribution to carbapenem resistance in Gram-negative bacilli. Bla<sub>AFM-1</sub> gene was first discovered by us in Alcaligenes faecalis AN70 strain isolated in Guangzhou of China and, was submitted to NCBI on 16 November 2018.<h4>Methods</h4>Antimicrobial susceptibility testing was performed by broth microdilution assay using BD Phoenix 100. The phylogenetic tree of AFM and other B1 metallo-β-lactamases was visualized by MEGA7.0. Whole-genome sequencing technology was used to sequence carbapenem-resistant strains including the bla<sub>AFM-1</sub> gene. Cloning and expressing of bla<sub>AFM-1</sub> were designed to verify the function of AFM-1 to hydrolyze carbapenems and common β-lactamase substrates. Carba NP and Etest experimen"],"journal":["Annals of clinical microbiology and antimicrobials"],"pubmed_title":["Characterization of bla<sub>AFM-1</sub>-positive carbapenem-resistant strains isolated in Guangzhou, China."],"pmcid":["PMC10189940"],"funding_grant_id":["2015A020211011","201510010167","2021A1515012249"],"pubmed_authors":["Peng Y","Rui Y","Qin Y","Huang R","Duan X","Song Z"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of bla<sub>AFM-1</sub>-positive carbapenem-resistant strains isolated in Guangzhou, China.","description":"<h4>Background</h4>Carbapenemase-producing makes a great contribution to carbapenem resistance in Gram-negative bacilli. Bla<sub>AFM-1</sub> gene was first discovered by us in Alcaligenes faecalis AN70 strain isolated in Guangzhou of China and, was submitted to NCBI on 16 November 2018.<h4>Methods</h4>Antimicrobial susceptibility testing was performed by broth microdilution assay using BD Phoenix 100. The phylogenetic tree of AFM and other B1 metallo-β-lactamases was visualized by MEGA7.0. Whole-genome sequencing technology was used to sequence carbapenem-resistant strains including the bla<sub>AFM-1</sub> gene. Cloning and expressing of bla<sub>AFM-1</sub> were designed to verify the function of AFM-1 to hydrolyze carbapenems and common β-lactamase substrates. Carba NP and Etest experimen","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-05-29T02:18:48.715Z","creation":"2025-02-19T02:23:09.545Z"},"accession":"S-EPMC10189940","cross_references":{"pubmed":["37198688"],"doi":["10.1186/s12941-023-00592-0"]}}