biostudies-literatureQin YGuangdong Province Science and Technology ProjectGuangzhou City Science and TechnologyNatural Science Foundation of Guangdong Province40https://www.ebi.ac.uk/biostudies/studies/S-EPMC10189940biostudies-literatureUnknown22(1)<h4>Background</h4>Carbapenemase-producing makes a great contribution to carbapenem resistance in Gram-negative bacilli. Bla_AFM-1 gene was first discovered by us in Alcaligenes faecalis AN70 strain isolated in Guangzhou of China and, was submitted to NCBI on 16 November 2018.<h4>Methods</h4>Antimicrobial susceptibility testing was performed by broth microdilution assay using BD Phoenix 100. The phylogenetic tree of AFM and other B1 metallo-β-lactamases was visualized by MEGA7.0. Whole-genome sequencing technology was used to sequence carbapenem-resistant strains including the bla_AFM-1 gene. Cloning and expressing of bla_AFM-1 were designed to verify the function of AFM-1 to hydrolyze carbapenems and common β-lactamase substrates. Carba NP and Etest experimenAnnals of clinical microbiology and antimicrobialsCharacterization of bla_AFM-1-positive carbapenem-resistant strains isolated in Guangzhou, China.PMC101899402015A0202110112015100101672021A1515012249Peng YRui YQin YHuang RDuan XSong ZfalseCharacterization of bla_AFM-1-positive carbapenem-resistant strains isolated in Guangzhou, China.<h4>Background</h4>Carbapenemase-producing makes a great contribution to carbapenem resistance in Gram-negative bacilli. Bla_AFM-1 gene was first discovered by us in Alcaligenes faecalis AN70 strain isolated in Guangzhou of China and, was submitted to NCBI on 16 November 2018.<h4>Methods</h4>Antimicrobial susceptibility testing was performed by broth microdilution assay using BD Phoenix 100. The phylogenetic tree of AFM and other B1 metallo-β-lactamases was visualized by MEGA7.0. Whole-genome sequencing technology was used to sequence carbapenem-resistant strains including the bla_AFM-1 gene. Cloning and expressing of bla_AFM-1 were designed to verify the function of AFM-1 to hydrolyze carbapenems and common β-lactamase substrates. Carba NP and Etest experimen2023-01-01T00:00:00Z2023 May2026-05-29T02:18:48.715Z2025-02-19T02:23:09.545ZS-EPMC101899403719868810.1186/s12941-023-00592-0