biostudies-literatureUnknown8(19)Chi XIn pursuit of developing novel azole antifungals with potent activity and high selectivity, a series of (2<i>R</i>,3<i>S</i>)-3-(substituted-1<i>H</i>-pyrazol-3-yl)-2-(2,4-difluorophenyl)-1-(1<i>H</i>-tetrazol-1-yl)butan-2-ol derivatives were designed and synthesized based on our previous work. All compounds exhibited excellent <i>in vitro</i> antifungal activities against <i>Candida</i> spp. and <i>Cryptococcus neoformans</i> H99 with minimum inhibitory concentration values ranging from <0.008 to 4 μg/mL, with some even showing moderate activity against <i>Aspergillus fumigatus</i> 7544. The most active compounds (<b>8</b>, <b>11</b>, <b>15</b>, <b>24</b>, and <b>25)</b> displayed outstanding antifungal activity against six fluconazole-resistant <i>C. auris</i> clinical isolates and showeACS omega17103-17115https://www.ebi.ac.uk/biostudies/studies/S-EPMC10193422biostudies-literatureDiscovery of Novel Tetrazoles Featuring a Pyrazole Moiety as Potent and Highly Selective Antifungal Agents.PMC10193422Jiang YLi XZhang HNi TChi XLi LWu HfalseDiscovery of Novel Tetrazoles Featuring a Pyrazole Moiety as Potent and Highly Selective Antifungal Agents.In pursuit of developing novel azole antifungals with potent activity and high selectivity, a series of (2<i>R</i>,3<i>S</i>)-3-(substituted-1<i>H</i>-pyrazol-3-yl)-2-(2,4-difluorophenyl)-1-(1<i>H</i>-tetrazol-1-yl)butan-2-ol derivatives were designed and synthesized based on our previous work. All compounds exhibited excellent <i>in vitro</i> antifungal activities against <i>Candida</i> spp. and <i>Cryptococcus neoformans</i> H99 with minimum inhibitory concentration values ranging from <0.008 to 4 μg/mL, with some even showing moderate activity against <i>Aspergillus fumigatus</i> 7544. The most active compounds (<b>8</b>, <b>11</b>, <b>15</b>, <b>24</b>, and <b>25)</b> displayed outstanding antifungal activity against six fluconazole-resistant <i>C. auris</i> clinical isolates and showe2023-01-01T00:00:00Z2023 May2025-04-26T21:30:36.745Z2025-04-06T16:47:43.654ZS-EPMC101934223721470610.1021/acsomega.3c01421