biostudies-literatureMolinas MSwiss National Science FoundationEuropean Research Council7537-7546https://www.ebi.ac.uk/biostudies/studies/S-EPMC10193582biostudies-literatureUnknown57(19)The biological reduction of soluble U(VI) complexes to form immobile U(IV) species has been proposed to remediate contaminated sites. It is well established that multiheme <i>c</i>-type cytochromes (MHCs) are key mediators of electron transfer to aqueous phase U(VI) complexes for bacteria such as <i>Shewanella oneidensis</i> MR-1. Recent studies have confirmed that the reduction proceeds via a first electron transfer forming pentavalent U(V) species that readily disproportionate. However, in the presence of the stabilizing aminocarboxylate ligand, dpaea^2- (dpaeaH₂═bis(pyridyl-6-methyl-2-carboxylate)-ethylamine), biologically produced U(V) persisted in aqueous solution at pH 7. We aim to pinpoint the role of MHC in the reduction of U(V)-dpaea and to establish the mechaEnvironmental science & technologyMechanism of Reduction of Aqueous U(V)-dpaea and Solid-Phase U(VI)-dpaea Complexes: The Role of Multiheme <i>c</i>-Type Cytochromes.PMC10193582200021E-164209725675Meibom KLMazzanti MBernier-Latmani RFaizova RMolinas MfalseMechanism of Reduction of Aqueous U(V)-dpaea and Solid-Phase U(VI)-dpaea Complexes: The Role of Multiheme <i>c</i>-Type Cytochromes.The biological reduction of soluble U(VI) complexes to form immobile U(IV) species has been proposed to remediate contaminated sites. It is well established that multiheme <i>c</i>-type cytochromes (MHCs) are key mediators of electron transfer to aqueous phase U(VI) complexes for bacteria such as <i>Shewanella oneidensis</i> MR-1. Recent studies have confirmed that the reduction proceeds via a first electron transfer forming pentavalent U(V) species that readily disproportionate. However, in the presence of the stabilizing aminocarboxylate ligand, dpaea^2- (dpaeaH₂═bis(pyridyl-6-methyl-2-carboxylate)-ethylamine), biologically produced U(V) persisted in aqueous solution at pH 7. We aim to pinpoint the role of MHC in the reduction of U(V)-dpaea and to establish the mecha2023-01-01T00:00:00Z2023 May2025-04-21T22:43:23.418Z2025-04-05T18:54:33.142ZS-EPMC101935823713383110.1021/acs.est.3c00666