{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14"],"submitter":["Kumar P"],"pubmed_abstract":["MicroRNAs are small non-coding RNAs that control gene expression during development, physiology, and disease. Transcription is a key factor in microRNA abundance and tissue-specific expression. Many databases predict the location of microRNA transcription start sites and promoters. However, these candidate regions require functional validation. Here, dCas9 fused to transcriptional activators or repressors - CRISPR activation (CRISPRa) and inhibition (CRISPRi)- were targeted to the candidate promoters of two intronic microRNAs, mmu-miR-335 and hsa-miR-3662, including the promoters of their respective host genes Mest and HBS1L. We report that in mouse embryonic stem cells and brain organoids, miR-335 was downregulated upon CRISPRi of its host gene Mest. Reciprocally, CRISPRa of Mest promoter"],"journal":["Frontiers in genetics"],"pagination":["1147222"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10196145"],"repository":["biostudies-literature"],"pubmed_title":["Functional mapping of microRNA promoters with dCas9 fused to transcriptional regulators."],"pmcid":["PMC10196145"],"pubmed_authors":["Lemmers C","Varrault A","Courtes M","Le Digarcher A","Monteil A","Bouschet T","Liu R","Kumar P","Coku I","Hong C","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"Functional mapping of microRNA promoters with dCas9 fused to transcriptional regulators.","description":"MicroRNAs are small non-coding RNAs that control gene expression during development, physiology, and disease. Transcription is a key factor in microRNA abundance and tissue-specific expression. Many databases predict the location of microRNA transcription start sites and promoters. However, these candidate regions require functional validation. Here, dCas9 fused to transcriptional activators or repressors - CRISPR activation (CRISPRa) and inhibition (CRISPRi)- were targeted to the candidate promoters of two intronic microRNAs, mmu-miR-335 and hsa-miR-3662, including the promoters of their respective host genes Mest and HBS1L. We report that in mouse embryonic stem cells and brain organoids, miR-335 was downregulated upon CRISPRi of its host gene Mest. Reciprocally, CRISPRa of Mest promoter","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-04T23:07:52.517Z","creation":"2025-04-04T23:07:52.517Z"},"accession":"S-EPMC10196145","cross_references":{"pubmed":["37214422"],"doi":["10.3389/fgene.2023.1147222"]}}