{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Casanovas-Massana A"],"funding":["National Institute of Allergy and Infectious Diseases","NIAID NIH HHS","FIC NIH HHS"],"pagination":["1450-1456"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10197099"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["70(3)"],"pubmed_abstract":["Four spirochetes (F1<sup>T</sup>, B21, Yale<sup>T</sup> and AMB6-RJ) were isolated from environmental sources: F1<sup>T</sup> and B21 from soils of an urban slum community in Salvador (Brazil), Yale<sup>T</sup> from river water in New Haven, Connecticut (USA) and AMB6-RJ from a pond in a horse farm in Rio de Janeiro (Brazil). Isolates were helix-shaped, aerobic, highly motile and non-virulent in a hamster model of infection. Draft genomes of the strains were obtained and analysed to determine the relatedness to other species of the genus <i>Leptospira</i>. The analysis of 498 core genes showed that strains F1<sup>T</sup>/B21 and Yale<sup>T</sup>/AMB6-RJ formed two distinct phylogenetic clades within the 'Pathogens' group (group I). The average nucleotide identity (ANI) values of strains F1<sup>T</sup>/B21 and Yale<sup>T</sup>/AMB6-RJ to other previously described <i>Leptospira</i> species were below <84 % and <82 %, respectively, which confirmed that these isolates should be classified as representatives of two novel species. Therefore, we propose <i>Leptospirayasudae</i> sp. nov. and <i>Leptospirastimsonii</i> sp. nov. as new species in the genus <i>Leptospira</i>. The type strains are F1<sup>T</sup> (=ATCC-TSD-163=KIT0259=CLEP00287) and Yale<sup>T</sup> (=ATCC-TDS-162=KIT0258=CLEP00288), respectively."],"journal":["International journal of systematic and evolutionary microbiology"],"pubmed_title":["<i>Leptospira yasudae</i> sp. nov. and <i>Leptospira stimsonii</i> sp. nov., two new species of the pathogenic group isolated from environmental sources."],"pmcid":["PMC10197099"],"funding_grant_id":["R25 TW009338","R01 AI121207","U01 AI088752","D43 TW010540","R01 AI052473","R01 TW009504"],"pubmed_authors":["Hacker KP","Medeiros MA","Hamond C","de Oliveira D","Casanovas-Massana A","Ko AI","Reis MG","Wunder EA","Costa F","Santos LA","Balassiano I"],"additional_accession":[]},"is_claimable":false,"name":"<i>Leptospira yasudae</i> sp. nov. and <i>Leptospira stimsonii</i> sp. nov., two new species of the pathogenic group isolated from environmental sources.","description":"Four spirochetes (F1<sup>T</sup>, B21, Yale<sup>T</sup> and AMB6-RJ) were isolated from environmental sources: F1<sup>T</sup> and B21 from soils of an urban slum community in Salvador (Brazil), Yale<sup>T</sup> from river water in New Haven, Connecticut (USA) and AMB6-RJ from a pond in a horse farm in Rio de Janeiro (Brazil). Isolates were helix-shaped, aerobic, highly motile and non-virulent in a hamster model of infection. Draft genomes of the strains were obtained and analysed to determine the relatedness to other species of the genus <i>Leptospira</i>. The analysis of 498 core genes showed that strains F1<sup>T</sup>/B21 and Yale<sup>T</sup>/AMB6-RJ formed two distinct phylogenetic clades within the 'Pathogens' group (group I). The average nucleotide identity (ANI) values of strains F1<sup>T</sup>/B21 and Yale<sup>T</sup>/AMB6-RJ to other previously described <i>Leptospira</i> species were below <84 % and <82 %, respectively, which confirmed that these isolates should be classified as representatives of two novel species. Therefore, we propose <i>Leptospirayasudae</i> sp. nov. and <i>Leptospirastimsonii</i> sp. nov. as new species in the genus <i>Leptospira</i>. The type strains are F1<sup>T</sup> (=ATCC-TSD-163=KIT0259=CLEP00287) and Yale<sup>T</sup> (=ATCC-TDS-162=KIT0258=CLEP00288), respectively.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Mar","modification":"2026-05-28T08:22:29.532Z","creation":"2025-04-05T21:08:31.44Z"},"accession":"S-EPMC10197099","cross_references":{"pubmed":["31184568"],"doi":["10.1099/ijsem.0.003480"]}}