{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chandramouly G"],"funding":["NCI NIH HHS","NIGMS NIH HHS"],"pagination":["107-114"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10197178"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["30(1)"],"pubmed_abstract":["The double-strand break (DSB) repair pathway called microhomology-mediated end-joining (MMEJ) is thought to be dependent on DNA polymerase theta (Polθ) and occur independently of nonhomologous end-joining (NHEJ) factors. An unresolved question is whether MMEJ is facilitated by a single Polθ-mediated end-joining pathway or consists of additional undiscovered pathways. We find that human X-family Polλ, which functions in NHEJ, additionally exhibits robust MMEJ activity like Polθ. Polλ promotes MMEJ in mammalian cells independently of essential NHEJ factors LIG4/XRCC4 and Polθ, which reveals a distinct Polλ-dependent MMEJ mechanism. X-ray crystallography employing in situ photo-induced DSB formation captured Polλ in the act of stabilizing a microhomology-mediated DNA synapse with incoming nuc"],"journal":["Nature structural & molecular biology"],"pubmed_title":["Polλ promotes microhomology-mediated end-joining."],"pmcid":["PMC10197178"],"funding_grant_id":["R01 GM130889","P30 CA006927","UH2 CA271230","R01 GM137124"],"pubmed_authors":["Pomerantz RT","Calbert ML","Tredinnick T","Demidova EV","Ozdemir AY","Tyagi M","Jamsen J","Wilson SH","Kent T","Borisonnik N","Chandramouly G","Arora S"],"additional_accession":[]},"is_claimable":false,"name":"Polλ promotes microhomology-mediated end-joining.","description":"The double-strand break (DSB) repair pathway called microhomology-mediated end-joining (MMEJ) is thought to be dependent on DNA polymerase theta (Polθ) and occur independently of nonhomologous end-joining (NHEJ) factors. An unresolved question is whether MMEJ is facilitated by a single Polθ-mediated end-joining pathway or consists of additional undiscovered pathways. We find that human X-family Polλ, which functions in NHEJ, additionally exhibits robust MMEJ activity like Polθ. Polλ promotes MMEJ in mammalian cells independently of essential NHEJ factors LIG4/XRCC4 and Polθ, which reveals a distinct Polλ-dependent MMEJ mechanism. X-ray crystallography employing in situ photo-induced DSB formation captured Polλ in the act of stabilizing a microhomology-mediated DNA synapse with incoming nuc","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-04T10:19:38.885Z","creation":"2025-02-19T00:12:15.933Z"},"accession":"S-EPMC10197178","cross_references":{"pubmed":["36536104"],"doi":["10.1038/s41594-022-00895-4"]}}