biostudies-literatureUnknownMorrison JJNIAID NIH HHSReducing growth and limiting metabolism are strategies that allow bacteria to survive exposure to environmental stress and antibiotics. During infection, uropathogenic <i>Escherichia coli</i> (UPEC) may enter a quiescent state that enables them to reemerge after completion of successful antibiotic treatment. Many clinical isolates, including the well characterized UPEC strain CFT073, also enter a metabolite-dependent, quiescent state in vitro that is reversible with cues, including peptidoglycan-derived peptides and amino acids. Here, we show that quiescent UPEC is antibiotic tolerant and demonstrate that metabolic flux in the tricarboxylic acid (TCA) cycle regulates the UPEC quiescent state via succinyl-CoA. We also demonstrate that the transcriptional regulator complex IHF and the FtsZ-ibioRxiv : the preprint server for biology2023.05.09.540013https://www.ebi.ac.uk/biostudies/studies/S-EPMC10197701biostudies-literatureMetabolic flux regulates growth transitions and antibiotic tolerance in uropathogenic <i>Escherichia coli</i>.PMC10197701R21 AI156574Cohen PSCamberg JLMadden EKBanas DADiBiasio ECRowley DCMorrison JJfalseMetabolic flux regulates growth transitions and antibiotic tolerance in uropathogenic <i>Escherichia coli</i>.Reducing growth and limiting metabolism are strategies that allow bacteria to survive exposure to environmental stress and antibiotics. During infection, uropathogenic <i>Escherichia coli</i> (UPEC) may enter a quiescent state that enables them to reemerge after completion of successful antibiotic treatment. Many clinical isolates, including the well characterized UPEC strain CFT073, also enter a metabolite-dependent, quiescent state in vitro that is reversible with cues, including peptidoglycan-derived peptides and amino acids. Here, we show that quiescent UPEC is antibiotic tolerant and demonstrate that metabolic flux in the tricarboxylic acid (TCA) cycle regulates the UPEC quiescent state via succinyl-CoA. We also demonstrate that the transcriptional regulator complex IHF and the FtsZ-i2023-01-01T00:00:00Z2023 May2025-04-21T21:48:56.117Z2025-04-21T21:48:56.117ZS-EPMC101977013721500210.1101/2023.05.09.540013