{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["169(2)"],"submitter":["Sah S"],"funding":["Science and Education Research Board, New Delhi, India","Tata Trusts (IISc), India","Department of Biotechnology, Ministry of Science and Technology, India"],"pubmed_abstract":["Methionyl-tRNA formyltransferase (Fmt)-mediated formylation of Met-tRNA<sup>fMet</sup> to fMet-tRNA<sup>fMet</sup> is crucial for efficient initiation of translation in bacteria and the eukaryotic organelles. Folate dehydrogenase-cyclohydrolase (FolD), a bifunctional enzyme, carries out conversion of 5,10-methylene tetrahydrofolate (5,10-CH<sub>2</sub>-THF) to 10-formyl-THF (10-CHO-THF), a metabolite utilized by Fmt as a formyl group donor. In this study, using <i>in vivo</i> and <i>in vitro</i> approaches, we show that 10-CHO-DHF may also be utilized by Fmt as an alternative substrate (formyl group donor) to formylate Met-tRNA<sup>fMet</sup>. Dihydrofolate (DHF) formed as a by-product in the <i>in vitro</i> assay was verified by LC-MS/MS analysis. FolD-deficient mutants and Fmt over-expre"],"journal":["Microbiology (Reading, England)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10197868"],"repository":["biostudies-literature"],"pubmed_title":["Methionyl-tRNA formyltransferase utilizes 10-formyldihydrofolate as an alternative substrate and impacts antifolate drug action."],"pmcid":["PMC10197868"],"pubmed_authors":["Sah S","Varshney U"],"additional_accession":[]},"is_claimable":false,"name":"Methionyl-tRNA formyltransferase utilizes 10-formyldihydrofolate as an alternative substrate and impacts antifolate drug action.","description":"Methionyl-tRNA formyltransferase (Fmt)-mediated formylation of Met-tRNA<sup>fMet</sup> to fMet-tRNA<sup>fMet</sup> is crucial for efficient initiation of translation in bacteria and the eukaryotic organelles. Folate dehydrogenase-cyclohydrolase (FolD), a bifunctional enzyme, carries out conversion of 5,10-methylene tetrahydrofolate (5,10-CH<sub>2</sub>-THF) to 10-formyl-THF (10-CHO-THF), a metabolite utilized by Fmt as a formyl group donor. In this study, using <i>in vivo</i> and <i>in vitro</i> approaches, we show that 10-CHO-DHF may also be utilized by Fmt as an alternative substrate (formyl group donor) to formylate Met-tRNA<sup>fMet</sup>. Dihydrofolate (DHF) formed as a by-product in the <i>in vitro</i> assay was verified by LC-MS/MS analysis. FolD-deficient mutants and Fmt over-expre","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-04T23:44:39.959Z","creation":"2025-02-19T03:55:29.849Z"},"accession":"S-EPMC10197868","cross_references":{"pubmed":["36745551"],"doi":["10.1099/mic.0.001297"]}}