{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gerlach K"],"funding":["DFG","Interdisciplinary Center for Clinical Research","Medical Research Council","German Research Council","Collaborative Research Centers","Emerging Fields Initiative and the Ludwig Demling Center"],"pagination":["1893-1910"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10197880"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(12)"],"pubmed_abstract":["Background and aims
Colorectal cancer [CRC] is one of the most frequent malignancies, but the molecular mechanisms driving cancer growth are incompletely understood. We characterised the roles of the cytokine IL-9 and Th9 cells in regulating CRC development.Methods
CRC patient samples and samples from AOM/DSS treated mice were analysed for expression of IL-9, CD3, and PU.1 by FACS analysis and immunohistochemistry. IL-9 citrine reporter mice, IL-9 knockout mice, and PU.1 and GATA3 CD4-Cre conditional knockout mice were studied in the AOM/DSS model. DNA minicircles or hyper-IL-6 were used for overexpression of cytokines in vivo. Effects of IL-6 and IL-9 were determined in organoid and T cell cultures. Claudin2/3 expression was studied by western blotting and bacterial transl"],"journal":["Journal of Crohn's & colitis"],"pubmed_title":["PU.1-driven Th9 Cells Promote Colorectal Cancer in Experimental Colitis Models Through Il-6 Effects in Intestinal Epithelial Cells."],"pmcid":["PMC10197880"],"funding_grant_id":["CRC 1181/2-B02","MC_U105178805","GE3043/3-1 [434401404]","WE4656/2-2"],"pubmed_authors":["Popp V","Atreya R","Dregelies T","Neurath MF","Gerlach K","Al-Saifi R","Rosenbauer F","Gonzalez Acera M","Fichtner-Feigl S","Vieth M","McKenzie ANJ","Wirtz S","Weigmann B"],"additional_accession":[]},"is_claimable":false,"name":"PU.1-driven Th9 Cells Promote Colorectal Cancer in Experimental Colitis Models Through Il-6 Effects in Intestinal Epithelial Cells.","description":"Background and aims
Colorectal cancer [CRC] is one of the most frequent malignancies, but the molecular mechanisms driving cancer growth are incompletely understood. We characterised the roles of the cytokine IL-9 and Th9 cells in regulating CRC development.Methods
CRC patient samples and samples from AOM/DSS treated mice were analysed for expression of IL-9, CD3, and PU.1 by FACS analysis and immunohistochemistry. IL-9 citrine reporter mice, IL-9 knockout mice, and PU.1 and GATA3 CD4-Cre conditional knockout mice were studied in the AOM/DSS model. DNA minicircles or hyper-IL-6 were used for overexpression of cytokines in vivo. Effects of IL-6 and IL-9 were determined in organoid and T cell cultures. Claudin2/3 expression was studied by western blotting and bacterial transl","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-21T22:46:22.617Z","creation":"2025-04-05T18:51:46.727Z"},"accession":"S-EPMC10197880","cross_references":{"pubmed":["35793807"],"doi":["10.1093/ecco-jcc/jjac097"]}}