biostudies-literatureWang SMinistry of Science and Technology of the People's Republic of ChinaGuangdong Key R & D ProjectDepartment of Education of Guangdong ProvinceShenzhen Special Fund for Sustainable Developmentthe Key Laboratory of Mariculture of Ministry of Education, Ocean University of ChinaShenzhen University 2035 InitiativeNational Natural Science Foundation of China84https://www.ebi.ac.uk/biostudies/studies/S-EPMC10199484biostudies-literatureUnknown16(1)<h4>Background</h4>Diatoms have been viewed as ideal cell factories for production of some high-value bioactive metabolites, such as fucoxanthin, but their applications are restrained by limited biomass yield. Mixotrophy, by using both CO₂ and organic carbon source, is believed effective to crack the bottleneck of biomass accumulation and achieve a sustainable bioproduct supply.<h4>Results</h4>Glycerol, among tested carbon sources, was proved as the sole that could significantly promote growth of Cylindrotheca sp. with illumination, a so-called growth pattern, mixotrophy. Biomass and fucoxanthin yields of Cylindrotheca sp., grown in medium with glycerol (2 g L^-1), was increased by 52% and 29%, respectively, as compared to the autotrophic culture (control) without compBiotechnology for biofuels and bioproductsTranscriptomic and metabolomic analyses revealed regulation mechanism of mixotrophic Cylindrotheca sp. glycerol utilization and biomass promotion.PMC101994842022B11110700052018YFA09025002022B0102022KQNCX06532273118KLM202204KCXFZ20211020164013021Zhao MHu ZWang SWu SZhou XfalseTranscriptomic and metabolomic analyses revealed regulation mechanism of mixotrophic Cylindrotheca sp. glycerol utilization and biomass promotion.<h4>Background</h4>Diatoms have been viewed as ideal cell factories for production of some high-value bioactive metabolites, such as fucoxanthin, but their applications are restrained by limited biomass yield. Mixotrophy, by using both CO₂ and organic carbon source, is believed effective to crack the bottleneck of biomass accumulation and achieve a sustainable bioproduct supply.<h4>Results</h4>Glycerol, among tested carbon sources, was proved as the sole that could significantly promote growth of Cylindrotheca sp. with illumination, a so-called growth pattern, mixotrophy. Biomass and fucoxanthin yields of Cylindrotheca sp., grown in medium with glycerol (2 g L^-1), was increased by 52% and 29%, respectively, as compared to the autotrophic culture (control) without comp2023-01-01T00:00:00Z2023 May2025-04-27T01:33:53.021Z2025-04-06T18:18:57.915ZS-EPMC101994843720869610.1186/s13068-023-02338-8