biostudies-literatureUnknown13(23)Bawa RA pyridine dicarboxylate Schiff-base DAS was synthesized for cascade colorimetric recognition of Ni²⁺ and PPi. The selectivity and sensitivity of chemosensor DAS was investigated through colorimetric and UV-vis studies in MeOH-PBS (5 : 1, v/v, pH = 7.4). The chemosensor formed a 2 : 1 complex with Ni²⁺ metal ions with a binding constant of <i>K</i>_a = 3.07 × 10³ M^-2. Besides, a plausible sensing mechanism is confirmed by single crystal X-ray diffraction (SC-XRD), Job's plot and Benesi-Hildebrand plot (B-H plot) experiments. Furthermore, the DAS-Ni²⁺ ensemble formed '<i>in situ</i>' was used to selectively recognise PPi. The limit of detection (LOD) of DAS for Ni²⁺ was found to be 0.14 μM and that of the DAS-Ni<supRSC advances15391-15400https://www.ebi.ac.uk/biostudies/studies/S-EPMC10201394biostudies-literatureA pyridine dicarboxylate based hydrazone Schiff base for reversible colorimetric recognition of Ni²⁺ and PPi.PMC10201394Pani BBawa RNegi SSingh BKumar RfalseA pyridine dicarboxylate based hydrazone Schiff base for reversible colorimetric recognition of Ni²⁺ and PPi.A pyridine dicarboxylate Schiff-base DAS was synthesized for cascade colorimetric recognition of Ni²⁺ and PPi. The selectivity and sensitivity of chemosensor DAS was investigated through colorimetric and UV-vis studies in MeOH-PBS (5 : 1, v/v, pH = 7.4). The chemosensor formed a 2 : 1 complex with Ni²⁺ metal ions with a binding constant of <i>K</i>_a = 3.07 × 10³ M^-2. Besides, a plausible sensing mechanism is confirmed by single crystal X-ray diffraction (SC-XRD), Job's plot and Benesi-Hildebrand plot (B-H plot) experiments. Furthermore, the DAS-Ni²⁺ ensemble formed '<i>in situ</i>' was used to selectively recognise PPi. The limit of detection (LOD) of DAS for Ni²⁺ was found to be 0.14 μM and that of the DAS-Ni<sup2023-01-01T00:00:00Z2023 May2025-04-29T09:12:09.544Z2025-04-06T19:17:29.555ZS-EPMC102013943722340810.1039/d3ra02021e