{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu CC"],"funding":["Ministry of Science and Technology","National Taiwan University"],"pagination":["4398-4414"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10201435"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["51(9)"],"pubmed_abstract":["The long non-coding telomeric RNA transcript TERRA, in the form of an RNA-DNA duplex, regulates telomere recombination. In a screen for nucleases that affects telomere recombination, mutations in DNA2, EXO1, MRE11 and SAE2 cause severe delay in type II survivor formation, indicating that type II telomere recombination is mediated through a mechanism similar to repairing double-strand breaks. On the other hand, mutation in RAD27 results in early formation of type II recombination, suggesting that RAD27 acts as a negative regulator in telomere recombination. RAD27 encodes a flap endonuclease that plays a role in DNA metabolism, including replication, repair and recombination. We demonstrate that Rad27 suppresses the accumulation of the TERRA-associated R-loop and selectively cleaves TERRA of"],"journal":["Nucleic acids research"],"pubmed_title":["Flap endonuclease Rad27 cleaves the RNA of R-loop structures to suppress telomere recombination."],"pmcid":["PMC10201435"],"funding_grant_id":["108L9014","MOST 111-2326-B-002-019","MOST107-2311-B-002-023-MY3","104R8955-2","MOST109-2311-B-002-014","NTU-CC-108L892004","MOST 111-2311-B-002-006-MY3"],"pubmed_authors":["Kao YW","Lei KH","Yu TY","Chi P","Liu CC","Lin JJ","Cheng TH","Chan HR","Su GC","Hsieh YZ","Kato T"],"additional_accession":[]},"is_claimable":false,"name":"Flap endonuclease Rad27 cleaves the RNA of R-loop structures to suppress telomere recombination.","description":"The long non-coding telomeric RNA transcript TERRA, in the form of an RNA-DNA duplex, regulates telomere recombination. In a screen for nucleases that affects telomere recombination, mutations in DNA2, EXO1, MRE11 and SAE2 cause severe delay in type II survivor formation, indicating that type II telomere recombination is mediated through a mechanism similar to repairing double-strand breaks. On the other hand, mutation in RAD27 results in early formation of type II recombination, suggesting that RAD27 acts as a negative regulator in telomere recombination. RAD27 encodes a flap endonuclease that plays a role in DNA metabolism, including replication, repair and recombination. We demonstrate that Rad27 suppresses the accumulation of the TERRA-associated R-loop and selectively cleaves TERRA of","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-26T13:00:14.96Z","creation":"2025-04-06T14:05:59.95Z"},"accession":"S-EPMC10201435","cross_references":{"pubmed":["36999631"],"doi":["10.1093/nar/gkad236"]}}