biostudies-literatureDasovich MJohns Hopkins UniversityNIA NIH HHSNational Institutes of HealthNIGMS NIH HHS1552-1572https://www.ebi.ac.uk/biostudies/studies/S-EPMC10202152biostudies-literatureUnknown83(10)PARPs catalyze ADP-ribosylation-a post-translational modification that plays crucial roles in biological processes, including DNA repair, transcription, immune regulation, and condensate formation. ADP-ribosylation can be added to a wide range of amino acids with varying lengths and chemical structures, making it a complex and diverse modification. Despite this complexity, significant progress has been made in developing chemical biology methods to analyze ADP-ribosylated molecules and their binding proteins on a proteome-wide scale. Additionally, high-throughput assays have been developed to measure the activity of enzymes that add or remove ADP-ribosylation, leading to the development of inhibitors and new avenues for therapy. Real-time monitoring of ADP-ribosylation dynamics can be achiMolecular cellPARPs and ADP-ribosylation: Deciphering the complexity with molecular tools.PMC10202152T32GM080189R01 GM104135T32 GM080189R01GM104135RF1AG071326RF1 AG071326Dasovich MLeung AKLfalsePARPs and ADP-ribosylation: Deciphering the complexity with molecular tools.PARPs catalyze ADP-ribosylation-a post-translational modification that plays crucial roles in biological processes, including DNA repair, transcription, immune regulation, and condensate formation. ADP-ribosylation can be added to a wide range of amino acids with varying lengths and chemical structures, making it a complex and diverse modification. Despite this complexity, significant progress has been made in developing chemical biology methods to analyze ADP-ribosylated molecules and their binding proteins on a proteome-wide scale. Additionally, high-throughput assays have been developed to measure the activity of enzymes that add or remove ADP-ribosylation, leading to the development of inhibitors and new avenues for therapy. Real-time monitoring of ADP-ribosylation dynamics can be achi2023-01-01T00:00:00Z2023 May2026-06-02T02:20:29.73Z2026-04-13T03:12:12.689ZS-EPMC102021523711981110.1016/j.molcel.2023.04.009