{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4(3)"],"submitter":["Ahmadli U"],"pubmed_abstract":["Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, haploid induction via mutation of the centromere-specific histone H3 (cenH3) has been shown when the mutant is outcrossed to the wild-type, and the wild-type genome remains in the haploid progeny. However, factors that affect haploid induction are still poorly understood. Here, we report that a mutant of the cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as a haploid inducer when pollinated by the wild-type. We discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction 10-fold. We also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines, suggestin"],"journal":["Plant communications"],"pagination":["100507"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10203384"],"repository":["biostudies-literature"],"pubmed_title":["High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis."],"pmcid":["PMC10203384"],"pubmed_authors":["Ahmadli U","Ingouff M","Lermontova I","Khaitova LC","Zuo S","Kalidass M","Heckmann S","Cuacos M","Mascher M","Fuchs J","Pecinkova J","Houben A","Riha K","Demidov D"],"additional_accession":[]},"is_claimable":false,"name":"High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis.","description":"Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, haploid induction via mutation of the centromere-specific histone H3 (cenH3) has been shown when the mutant is outcrossed to the wild-type, and the wild-type genome remains in the haploid progeny. However, factors that affect haploid induction are still poorly understood. Here, we report that a mutant of the cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as a haploid inducer when pollinated by the wild-type. We discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction 10-fold. We also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines, suggestin","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-05-28T17:51:06.278Z","creation":"2025-04-04T14:29:50.783Z"},"accession":"S-EPMC10203384","cross_references":{"pubmed":["36540022"],"doi":["10.1016/j.xplc.2022.100507"]}}