biostudies-literatureUnknown4(3)Ahmadli UDouble haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, haploid induction via mutation of the centromere-specific histone H3 (cenH3) has been shown when the mutant is outcrossed to the wild-type, and the wild-type genome remains in the haploid progeny. However, factors that affect haploid induction are still poorly understood. Here, we report that a mutant of the cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as a haploid inducer when pollinated by the wild-type. We discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction 10-fold. We also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines, suggestinPlant communications100507https://www.ebi.ac.uk/biostudies/studies/S-EPMC10203384biostudies-literatureHigh temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis.PMC10203384Ahmadli UIngouff MLermontova IKhaitova LCZuo SKalidass MHeckmann SCuacos MMascher MFuchs JPecinkova JHouben ARiha KDemidov DfalseHigh temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis.Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, haploid induction via mutation of the centromere-specific histone H3 (cenH3) has been shown when the mutant is outcrossed to the wild-type, and the wild-type genome remains in the haploid progeny. However, factors that affect haploid induction are still poorly understood. Here, we report that a mutant of the cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as a haploid inducer when pollinated by the wild-type. We discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction 10-fold. We also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines, suggestin2023-01-01T00:00:00Z2023 May2026-05-28T17:51:06.278Z2025-04-04T14:29:50.783ZS-EPMC102033843654002210.1016/j.xplc.2022.100507