biostudies-literatureUnknown13(1)Kielholz TJohann Wolfgang Goethe-Universität, Frankfurt am MainThe increasing incidence of infected skin wounds poses a major challenge in clinical practice, especially when conventional antibiotic therapy fails. In this context, bacteriophages emerged as promising alternatives for the treatment of antibiotic-resistant bacteria. However, clinical implementation remains hampered by the lack of efficient delivery approaches to infected wound tissue. In this study, bacteriophage-loaded electrospun fiber mats were successfully developed as next-generation wound dressings for the treatment of infected wounds. We employed a coaxial electrospinning approach, creating fibers with a protective polymer shell, enveloping bacteriophages in the core while maintaining their antimicrobial activity. The novel fibers exhibited a reproducible fiber diameter range and mScientific reports8330https://www.ebi.ac.uk/biostudies/studies/S-EPMC10205809biostudies-literatureBacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections.PMC10205809Rohde FJung NWindbergs MKielholz TfalseBacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections.The increasing incidence of infected skin wounds poses a major challenge in clinical practice, especially when conventional antibiotic therapy fails. In this context, bacteriophages emerged as promising alternatives for the treatment of antibiotic-resistant bacteria. However, clinical implementation remains hampered by the lack of efficient delivery approaches to infected wound tissue. In this study, bacteriophage-loaded electrospun fiber mats were successfully developed as next-generation wound dressings for the treatment of infected wounds. We employed a coaxial electrospinning approach, creating fibers with a protective polymer shell, enveloping bacteriophages in the core while maintaining their antimicrobial activity. The novel fibers exhibited a reproducible fiber diameter range and m2023-01-01T00:00:00Z2023 May2025-04-06T19:16:38.357Z2025-04-06T19:16:38.357ZS-EPMC102058093722119410.1038/s41598-023-35364-5