{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ebata H"],"funding":["Japan Society for the Promotion of Science"],"pagination":["1781-1793"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10209042"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["122(10)"],"pubmed_abstract":["Active microrheology was conducted in living cells by applying an optical-trapping force to vigorously fluctuating tracer beads with feedback-tracking technology. The complex shear modulus G(ω)=G<sup>'</sup>(ω)-iG<sup>″</sup>(ω) was measured in HeLa cells in an epithelial-like confluent monolayer. We found that G(ω)∝(-iω)<sup>1/2</sup> over a wide range of frequencies (1 Hz < ω/2π < 10 kHz). Actin disruption and cell-cycle progression from G1 to S and G2 phases only had a limited effect on G(ω) in living cells. On the other hand, G(ω) was found to be dependent on cell metabolism; ATP-depleted cells showed an increased elastic modulus G<sup>'</sup>(ω) at low frequencies, giving rise to a constant plateau such that G(ω)=G<sub>0</sub>+A(-iω)<sup>1/2</sup>. Both the plateau and the additional frequency dependency ∝(-iω)<sup>1/2</sup> of ATP-depleted cells are consistent with a rheological response typical of colloidal jamming. On the other hand, the plateau G<sub>0</sub> disappeared in ordinary metabolically active cells, implying that living cells fluidize their internal states such that they approach the critical jamming point."],"journal":["Biophysical journal"],"pubmed_title":["Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology."],"pmcid":["PMC10209042"],"funding_grant_id":["JP21H01048","JP20H05536","JP20H00128","JP22H04848"],"pubmed_authors":["Mizuno D","Inokuchi S","Miyamoto T","Nishizawa K","Umeda K","Sugino Y","Ebata H","Nagao W"],"additional_accession":[]},"is_claimable":false,"name":"Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology.","description":"Active microrheology was conducted in living cells by applying an optical-trapping force to vigorously fluctuating tracer beads with feedback-tracking technology. The complex shear modulus G(ω)=G<sup>'</sup>(ω)-iG<sup>″</sup>(ω) was measured in HeLa cells in an epithelial-like confluent monolayer. We found that G(ω)∝(-iω)<sup>1/2</sup> over a wide range of frequencies (1 Hz < ω/2π < 10 kHz). Actin disruption and cell-cycle progression from G1 to S and G2 phases only had a limited effect on G(ω) in living cells. On the other hand, G(ω) was found to be dependent on cell metabolism; ATP-depleted cells showed an increased elastic modulus G<sup>'</sup>(ω) at low frequencies, giving rise to a constant plateau such that G(ω)=G<sub>0</sub>+A(-iω)<sup>1/2</sup>. Both the plateau and the additional frequency dependency ∝(-iω)<sup>1/2</sup> of ATP-depleted cells are consistent with a rheological response typical of colloidal jamming. On the other hand, the plateau G<sub>0</sub> disappeared in ordinary metabolically active cells, implying that living cells fluidize their internal states such that they approach the critical jamming point.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-06-27T03:05:21.551Z","creation":"2025-06-27T03:05:21.551Z"},"accession":"S-EPMC10209042","cross_references":{"pubmed":["37050875"],"doi":["10.1016/j.bpj.2023.04.011"]}}