{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang H"],"funding":["National Key Research and Development Program of China","National Natural Science Foundation (NSFC) of China"],"pagination":["168"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10210397"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Background
Giardia duodenalis (referred to as Giardia) is a flagellated binucleate protozoan parasite, which causes one of the most common diarrheal diseases, giardiasis, worldwide. Giardia can be infected by Giardiavirus (GLV), a small endosymbiotic dsRNA virus belongs to the Totiviridae family. However, the regulation of GLV and a positive correlation between GLV and Giardia virulence is yet to be elucidated.Methods
To identify potential regulators of GLV, we performed a yeast two-hybrid (Y2H) screen to search for interacting proteins of RdRp. GST pull-down, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assay were used to verify the direct physical interaction between GLV RdRp and its new binding partner. In addition, their in vivo interaction"],"journal":["Parasites & vectors"],"pubmed_title":["A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication."],"pmcid":["PMC10210397"],"funding_grant_id":["31972704","No. 2021YFF0702900"],"pubmed_authors":["Cheng S","Li X","Yue T","Li J","Zhao C","Gong P","Zhang H","Zhang X","Zhang N","Wang X"],"additional_accession":[]},"is_claimable":false,"name":"A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication.","description":"Background
Giardia duodenalis (referred to as Giardia) is a flagellated binucleate protozoan parasite, which causes one of the most common diarrheal diseases, giardiasis, worldwide. Giardia can be infected by Giardiavirus (GLV), a small endosymbiotic dsRNA virus belongs to the Totiviridae family. However, the regulation of GLV and a positive correlation between GLV and Giardia virulence is yet to be elucidated.Methods
To identify potential regulators of GLV, we performed a yeast two-hybrid (Y2H) screen to search for interacting proteins of RdRp. GST pull-down, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assay were used to verify the direct physical interaction between GLV RdRp and its new binding partner. In addition, their in vivo interaction","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-22T01:12:08.642Z","creation":"2025-04-05T19:49:35.152Z"},"accession":"S-EPMC10210397","cross_references":{"pubmed":["37226181"],"doi":["10.1186/s13071-023-05787-0"]}}