biostudies-literatureZhang HNational Key Research and Development Program of ChinaNational Natural Science Foundation (NSFC) of China168https://www.ebi.ac.uk/biostudies/studies/S-EPMC10210397biostudies-literatureUnknown16(1)<h4>Background</h4>Giardia duodenalis (referred to as Giardia) is a flagellated binucleate protozoan parasite, which causes one of the most common diarrheal diseases, giardiasis, worldwide. Giardia can be infected by Giardiavirus (GLV), a small endosymbiotic dsRNA virus belongs to the Totiviridae family. However, the regulation of GLV and a positive correlation between GLV and Giardia virulence is yet to be elucidated.<h4>Methods</h4>To identify potential regulators of GLV, we performed a yeast two-hybrid (Y2H) screen to search for interacting proteins of RdRp. GST pull-down, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assay were used to verify the direct physical interaction between GLV RdRp and its new binding partner. In addition, their in vivo interactionParasites & vectorsA potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication.PMC1021039731972704No. 2021YFF0702900Cheng SLi XYue TLi JZhao CGong PZhang HZhang XZhang NWang XfalseA potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication.<h4>Background</h4>Giardia duodenalis (referred to as Giardia) is a flagellated binucleate protozoan parasite, which causes one of the most common diarrheal diseases, giardiasis, worldwide. Giardia can be infected by Giardiavirus (GLV), a small endosymbiotic dsRNA virus belongs to the Totiviridae family. However, the regulation of GLV and a positive correlation between GLV and Giardia virulence is yet to be elucidated.<h4>Methods</h4>To identify potential regulators of GLV, we performed a yeast two-hybrid (Y2H) screen to search for interacting proteins of RdRp. GST pull-down, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assay were used to verify the direct physical interaction between GLV RdRp and its new binding partner. In addition, their in vivo interaction2023-01-01T00:00:00Z2023 May2025-04-22T01:12:08.642Z2025-04-05T19:49:35.152ZS-EPMC102103973722618110.1186/s13071-023-05787-0