biostudies-literatureUnknown14Liao Z<h4>Background</h4>The emergence of multi-drug resistant <i>Staphylococcus aureus</i> (<i>S. aureus</i>) has posed a challenging clinical problem for treating its infection. The development of novel or new antibacterial agents becomes one of the useful methods to solve this problem, and has received more attention over the past decade. Citral is reported to have antibacterial activity against <i>S. aureus</i>, but its mechanism is yet entirely clear.<h4>Methods</h4>To reveal the antibacterial mechanism of citral against <i>S. aureus</i>, comparative transcriptomic analysis was carried out to analyze the gene expression differences between the citral-treated and untreated groups. The changes of protein, adenosine triphosphate (ATP) and reactive oxygen species (ROS) content in <i>S. aureus</Frontiers in microbiology1171339https://www.ebi.ac.uk/biostudies/studies/S-EPMC10213633biostudies-literatureTranscriptomic analyses reveal the potential antibacterial mechanism of citral against <i>Staphylococcus aureus</i>.PMC10213633Liao ZDai MXie YShao YLiao WYu GSun FLin KfalseTranscriptomic analyses reveal the potential antibacterial mechanism of citral against <i>Staphylococcus aureus</i>.<h4>Background</h4>The emergence of multi-drug resistant <i>Staphylococcus aureus</i> (<i>S. aureus</i>) has posed a challenging clinical problem for treating its infection. The development of novel or new antibacterial agents becomes one of the useful methods to solve this problem, and has received more attention over the past decade. Citral is reported to have antibacterial activity against <i>S. aureus</i>, but its mechanism is yet entirely clear.<h4>Methods</h4>To reveal the antibacterial mechanism of citral against <i>S. aureus</i>, comparative transcriptomic analysis was carried out to analyze the gene expression differences between the citral-treated and untreated groups. The changes of protein, adenosine triphosphate (ATP) and reactive oxygen species (ROS) content in <i>S. aureus</2023-01-01T00:00:00Z20232025-04-22T21:58:55.263Z2025-04-06T03:53:22.029ZS-EPMC102136333725003210.3389/fmicb.2023.1171339