biostudies-literatureYu QNational Natural Science Foundation of China1127https://www.ebi.ac.uk/biostudies/studies/S-EPMC10215317biostudies-literatureUnknown12(5)Hydrogen sulfide (H₂S) and its oxidation product zero-valent sulfur (S⁰) play important roles in animals, plants, and bacteria. Inside cells, S⁰ exists in various forms, including polysulfide and persulfide, which are collectively referred to as sulfane sulfur. Due to the known health benefits, the donors of H₂S and sulfane sulfur have been developed and tested. Among them, thiosulfate is a known H₂S and sulfane sulfur donor. We have previously reported that thiosulfate is an effective sulfane sulfur donor in <i>Escherichia coli</i>; however, it is unclear how it converts thiosulfate to cellular sulfane sulfur. In this study, we showed that one of the various rhodaneses, PspE, in <i>E. coli</i> was responsible for the conversion. AfterAntioxidants (Basel, Switzerland)The Rhodanese PspE Converts Thiosulfate to Cellular Sulfane Sulfur in <i>Escherichia coli</i>.PMC1021531731870085Liu HXia YXun LYu QRan MXin YfalseThe Rhodanese PspE Converts Thiosulfate to Cellular Sulfane Sulfur in <i>Escherichia coli</i>.Hydrogen sulfide (H₂S) and its oxidation product zero-valent sulfur (S⁰) play important roles in animals, plants, and bacteria. Inside cells, S⁰ exists in various forms, including polysulfide and persulfide, which are collectively referred to as sulfane sulfur. Due to the known health benefits, the donors of H₂S and sulfane sulfur have been developed and tested. Among them, thiosulfate is a known H₂S and sulfane sulfur donor. We have previously reported that thiosulfate is an effective sulfane sulfur donor in <i>Escherichia coli</i>; however, it is unclear how it converts thiosulfate to cellular sulfane sulfur. In this study, we showed that one of the various rhodaneses, PspE, in <i>E. coli</i> was responsible for the conversion. After2023-01-01T00:00:00Z2023 May2025-04-05T14:15:16.164Z2025-04-05T14:15:16.164ZS-EPMC102153173723799310.3390/antiox12051127