biostudies-literatureThevendran RTechnology Development Fund 1 grant of Ministry of Science, Technology and Innovation722https://www.ebi.ac.uk/biostudies/studies/S-EPMC10215423biostudies-literatureUnknown12(5)Dengue infection, caused by the dengue virus, is a global threat which requires immediate attention and appropriate disease management. The current diagnosis of dengue infection is largely based on viral isolation, RT-PCR and serology-based detection, which are time-consuming and expensive, and require trained personnel. For early diagnosis of dengue, the direct detection of a dengue antigenic target is efficacious, and one such target is NS1. NS1-based detection is primarily antibody-centric and is beset by drawbacks pertaining to antibodies such as the high cost of synthesis and large batch-to-batch variation. Aptamers are potential surrogates of antibodies and are much cheaper, without exhibiting batch-to-batch variation. Given these advantages, we sought to isolate RNA aptamers againstBiologyThe Diagnostic Potential of RNA Aptamers against the NS1 Protein of Dengue Virus Serotype 2.PMC10215423TDF03211027Leighton GTang THCitartan MMutombwera AThevendran RRogini SShigdar SfalseThe Diagnostic Potential of RNA Aptamers against the NS1 Protein of Dengue Virus Serotype 2.Dengue infection, caused by the dengue virus, is a global threat which requires immediate attention and appropriate disease management. The current diagnosis of dengue infection is largely based on viral isolation, RT-PCR and serology-based detection, which are time-consuming and expensive, and require trained personnel. For early diagnosis of dengue, the direct detection of a dengue antigenic target is efficacious, and one such target is NS1. NS1-based detection is primarily antibody-centric and is beset by drawbacks pertaining to antibodies such as the high cost of synthesis and large batch-to-batch variation. Aptamers are potential surrogates of antibodies and are much cheaper, without exhibiting batch-to-batch variation. Given these advantages, we sought to isolate RNA aptamers against2023-01-01T00:00:00Z2023 May2025-04-04T19:29:47.684Z2025-04-04T19:29:47.684ZS-EPMC102154233723753610.3390/biology12050722