biostudies-literatureDai WResearch Grants Council of the Hong Kong Special Administrative Region, ChinaEnvironment and Conservation Fund (ECF) of Hong Kong SAR, China523https://www.ebi.ac.uk/biostudies/studies/S-EPMC10216052biostudies-literatureUnknown13(5)Biological toxicity testing plays an essential role in identifying the possible negative effects induced by substances such as organic pollutants or heavy metals. As an alternative to conventional methods of toxicity detection, paper-based analytical device (PAD) offers advantages in terms of convenience, quick results, environmental friendliness, and cost-effectiveness. However, detecting the toxicity of both organic pollutants and heavy metals is challenging for a PAD. Here, we show the evaluation of biotoxicity testing for chlorophenols (pentachlorophenol, 2,4-dichlorophenol, and 4-chlorophenol) and heavy metals (Cu²⁺, Zn²⁺, and Pb²⁺) by a resazurin-integrated PAD. The results were achieved by observing the colourimetric response of bacteria (<i>EnterocoBiosensorsA Dye-Assisted Paper-Based Assay to Rapidly Differentiate the Stress of Chlorophenols and Heavy Metals on <i>Enterococcus faecalis</i> and <i>Escherichia coli</i>.PMC10216052ECF 02/2019UGC/FDS16/M(P)05/21Yau YDai WMao GSarmiento AHan JWong PYPeng YKInumbra BChen JLfalseA Dye-Assisted Paper-Based Assay to Rapidly Differentiate the Stress of Chlorophenols and Heavy Metals on <i>Enterococcus faecalis</i> and <i>Escherichia coli</i>.Biological toxicity testing plays an essential role in identifying the possible negative effects induced by substances such as organic pollutants or heavy metals. As an alternative to conventional methods of toxicity detection, paper-based analytical device (PAD) offers advantages in terms of convenience, quick results, environmental friendliness, and cost-effectiveness. However, detecting the toxicity of both organic pollutants and heavy metals is challenging for a PAD. Here, we show the evaluation of biotoxicity testing for chlorophenols (pentachlorophenol, 2,4-dichlorophenol, and 4-chlorophenol) and heavy metals (Cu²⁺, Zn²⁺, and Pb²⁺) by a resazurin-integrated PAD. The results were achieved by observing the colourimetric response of bacteria (<i>Enteroco2023-01-01T00:00:00Z2023 May2025-04-04T19:30:05.717Z2024-11-13T01:37:17.643ZS-EPMC102160523723288410.3390/bios13050523