{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(10)"],"submitter":[null],"funding":["National Research Council of Thailand"],"pubmed_abstract":["Previously established immune-responsive co-culture models with macrophages have limitations due to the dedifferentiation of macrophages in long-term cultures. This study is the first report of a long-term (21-day) triple co-culture of THP-1 macrophages (THP-1m) with Caco-2 intestinal epithelial cells and HT-29-methotrexate (MTX) goblet cells. We demonstrated that high-density seeded THP-1 cells treated with 100 ng/mL phorbol 12-myristate 13-acetate for 48 h differentiated stably and could be cultured for up to 21 days. THP-1m were identified by their adherent morphology and lysosome expansion. In the triple co-culture immune-responsive model, cytokine secretions during lipopolysaccharide-induced inflammation were confirmed. Tumor necrosis factor-alpha and interleukin 6 levels were elevate"],"journal":["Cells"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10217152"],"repository":["biostudies-literature"],"pubmed_title":["Optimizing THP-1 Macrophage Culture for an Immune-Responsive Human Intestinal Model"],"pmcid":["PMC10217152"],"funding_grant_id":["NRCT 105/2561"],"additional_accession":[]},"is_claimable":false,"name":"Optimizing THP-1 Macrophage Culture for an Immune-Responsive Human Intestinal Model","description":"Previously established immune-responsive co-culture models with macrophages have limitations due to the dedifferentiation of macrophages in long-term cultures. This study is the first report of a long-term (21-day) triple co-culture of THP-1 macrophages (THP-1m) with Caco-2 intestinal epithelial cells and HT-29-methotrexate (MTX) goblet cells. We demonstrated that high-density seeded THP-1 cells treated with 100 ng/mL phorbol 12-myristate 13-acetate for 48 h differentiated stably and could be cultured for up to 21 days. THP-1m were identified by their adherent morphology and lysosome expansion. In the triple co-culture immune-responsive model, cytokine secretions during lipopolysaccharide-induced inflammation were confirmed. Tumor necrosis factor-alpha and interleukin 6 levels were elevate","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-19T22:59:54.199Z","creation":"2025-04-19T22:59:54.199Z"},"accession":"S-EPMC10217152","cross_references":{}}