biostudies-literatureSanchez-Estrada RSecretaría de Investigación y Posgrado, Instituto Politécnico Nacional (IPN)Consejo Nacional de Ciencia y Tecnología (CONACyT), México1023https://www.ebi.ac.uk/biostudies/studies/S-EPMC10218544biostudies-literatureUnknown14(5)The slow-growing, nontuberculous mycobacterium <i>Mycobacterium kumamotonense</i> possesses two rRNA operons, <i>rrnA</i> and <i>rrnB</i>, located downstream from the <i>murA</i> and <i>tyrS</i> genes, respectively. Here, we report the sequence and organization of the promoter regions of these two <i>rrn</i> operons. In the <i>rrnA</i> operon, transcription can be initiated from the two promoters, named P1 <i>rrnA</i> and PCL1, while in <i>rrnB</i>, transcription can only start from one, called P1 <i>rrnB</i>. Both <i>rrn</i> operons show a similar organization to the one described in <i>Mycobacterium celatum</i> and <i>Mycobacterium smegmatis</i>. Furthermore, by qRT-PCR analyses of the products generated from each promoter, we report that stress conditions such as starvation, hypoxia, anGenesOrganization and Characterization of the Promoter Elements of the rRNA Operons in the Slow-Growing Pathogen <i>Mycobacterium kumamotonense</i>.PMC10218544SIP20231561 (SR-G)CB-2015-01-255181 (SR-G)SIP20230257 (JFC-C)Gonzalez-Y-Merchand JAGarcia-Morales LCerna-Cortes JFSanchez-Estrada RRivera-Gutierrez SMenendez MCLeon-Solis LEGarcia MJMendez-Guerrero OfalseOrganization and Characterization of the Promoter Elements of the rRNA Operons in the Slow-Growing Pathogen <i>Mycobacterium kumamotonense</i>.The slow-growing, nontuberculous mycobacterium <i>Mycobacterium kumamotonense</i> possesses two rRNA operons, <i>rrnA</i> and <i>rrnB</i>, located downstream from the <i>murA</i> and <i>tyrS</i> genes, respectively. Here, we report the sequence and organization of the promoter regions of these two <i>rrn</i> operons. In the <i>rrnA</i> operon, transcription can be initiated from the two promoters, named P1 <i>rrnA</i> and PCL1, while in <i>rrnB</i>, transcription can only start from one, called P1 <i>rrnB</i>. Both <i>rrn</i> operons show a similar organization to the one described in <i>Mycobacterium celatum</i> and <i>Mycobacterium smegmatis</i>. Furthermore, by qRT-PCR analyses of the products generated from each promoter, we report that stress conditions such as starvation, hypoxia, an2023-01-01T00:00:00Z2023 Apr2026-04-08T12:33:42.394Z2025-04-19T15:07:05.667ZS-EPMC102185443723938410.3390/genes14051023