biostudies-literatureJung YWKorea Forest Service (Korea Forestry Promotion Institute)8866https://www.ebi.ac.uk/biostudies/studies/S-EPMC10218641biostudies-literatureUnknown24(10)<i>Smilax sieboldii</i>, a climbing tree belonging to Smilacaceae, has been used in traditional oriental medicine for treating arthritis, tumors, leprosy, psoriasis, and lumbago. To evaluate the anti-obesity effects of <i>S. sieboldii</i> (Smilacaceae), we screened methylene chloride (CH₂Cl₂), ethyl acetate (EtOAc), aqueous-saturated <i>n</i>-butanol, and ethanol (EtOH) extracts of the whole plant at various concentrations to inhibit adipogenesis in adipocytes. The 3T3-L1 cell line with Oil red O staining with the help of fluorometry was used as an indicator of anti-obesity activity. Bioactivity-guided fractionation of the EtOH extract and subsequent phytochemical investigation of the active CH₂Cl₂- and EtOAc-soluble fractions resulted in the isoInternational journal of molecular sciencesAnti-Adipogenic Activity of Secondary Metabolites Isolated from <i>Smilax sieboldii</i> Miq. on 3T3-L1 Adipocytes.PMC102186412017027A00-1719-BA01Choi CWLee JEJeong WCha HChoi YHLee JAAhn EKHong SSOh JSJung YWfalseAnti-Adipogenic Activity of Secondary Metabolites Isolated from <i>Smilax sieboldii</i> Miq. on 3T3-L1 Adipocytes.<i>Smilax sieboldii</i>, a climbing tree belonging to Smilacaceae, has been used in traditional oriental medicine for treating arthritis, tumors, leprosy, psoriasis, and lumbago. To evaluate the anti-obesity effects of <i>S. sieboldii</i> (Smilacaceae), we screened methylene chloride (CH₂Cl₂), ethyl acetate (EtOAc), aqueous-saturated <i>n</i>-butanol, and ethanol (EtOH) extracts of the whole plant at various concentrations to inhibit adipogenesis in adipocytes. The 3T3-L1 cell line with Oil red O staining with the help of fluorometry was used as an indicator of anti-obesity activity. Bioactivity-guided fractionation of the EtOH extract and subsequent phytochemical investigation of the active CH₂Cl₂- and EtOAc-soluble fractions resulted in the iso2023-01-01T00:00:00Z2023 May2025-04-05T14:15:31.031Z2025-04-05T14:15:31.031ZS-EPMC102186413724021210.3390/ijms24108866