biostudies-literatureMori da Cunha MGMCFonds Wetenschappelijk Onderzoek8622https://www.ebi.ac.uk/biostudies/studies/S-EPMC10224946biostudies-literatureUnknown13(1)Vaginal birth causes pelvic floor injury which may lead to urinary incontinence. Cell therapy has been proposed to assist in functional recovery. We aim to assess if intra-arterial injection of rat mesoangioblasts (MABs) and stable Vascular Endothelial Growth Factor (VEGF)-expressing MABs, improve recovery of urethral and vaginal function following simulated vaginal delivery (SVD). Female rats (n = 86) were assigned to either injection of saline (control), allogeneic-MABs (MABs^allo), autologous-MABs (MABs^auto) or allogeneic-MABs transduced to stably expressed VEGF (MABs^allo-VEGF). One hour after SVD, 0.5 × 10⁶ MABs or saline were injected into the aorta. Primary outcome was urethral (7d and 14d) and vaginal (14d) function; others were bioluminescScientific reportsVEGF overexpressed mesoangioblasts enhance urethral and vaginal recovery following simulated vaginal birth in rats.PMC1022494611E7920NG069715Nvan der Veer BKCattani LMackova KKoh KPDeprest JSampaolesi MMori da Cunha MGMCVande Velde GGijsbers RAlbersen MGiacomazzi GfalseVEGF overexpressed mesoangioblasts enhance urethral and vaginal recovery following simulated vaginal birth in rats.Vaginal birth causes pelvic floor injury which may lead to urinary incontinence. Cell therapy has been proposed to assist in functional recovery. We aim to assess if intra-arterial injection of rat mesoangioblasts (MABs) and stable Vascular Endothelial Growth Factor (VEGF)-expressing MABs, improve recovery of urethral and vaginal function following simulated vaginal delivery (SVD). Female rats (n = 86) were assigned to either injection of saline (control), allogeneic-MABs (MABs^allo), autologous-MABs (MABs^auto) or allogeneic-MABs transduced to stably expressed VEGF (MABs^allo-VEGF). One hour after SVD, 0.5 × 10⁶ MABs or saline were injected into the aorta. Primary outcome was urethral (7d and 14d) and vaginal (14d) function; others were bioluminesc2023-01-01T00:00:00Z2023 May2025-04-04T09:18:48.265Z2025-04-04T09:18:48.265ZS-EPMC102249463724497510.1038/s41598-023-35809-x