biostudies-literatureLi CNational Major Science and Technology Projects of ChinaNational Natural Science Foundation of China1154-1167https://www.ebi.ac.uk/biostudies/studies/S-EPMC10225488biostudies-literatureUnknown20(6)The rapid spread of carbapenemase-producing Klebsiella pneumoniae (cpKP) poses serious threats to public health; however, the underlying genetic basis for its dissemination is still unknown. We conducted a comprehensive genomic epidemiology analysis on 420 cpKP isolates collected from 70 hospitals in 24 provinces/autonomous regions/municipalities of China during 2009-2017 by short-/long-read sequencing. The results showed that most cpKP isolates were categorized into clonal group 258 (CG258), in which ST11 was the dominant clone. Phylogenetic analysis revealed three major clades including the top one of Clade 3 for CG258 cpKP isolates. Additionally, carbapenemase gene analysis indicated that bla_KPC was dominant in the cpKP isolates, and most bla_KPC genes were located Genomics, proteomics & bioinformaticsGenomic Epidemiology of Carbapenemase-producing Klebsiella pneumoniae in China.PMC102254882018ZX10302-301-004-00331770870Li CJu YYue LChen FYin ZJing YYang XLi SJiang XZhou DWang XLuo XYang TMa GfalseGenomic Epidemiology of Carbapenemase-producing Klebsiella pneumoniae in China.The rapid spread of carbapenemase-producing Klebsiella pneumoniae (cpKP) poses serious threats to public health; however, the underlying genetic basis for its dissemination is still unknown. We conducted a comprehensive genomic epidemiology analysis on 420 cpKP isolates collected from 70 hospitals in 24 provinces/autonomous regions/municipalities of China during 2009-2017 by short-/long-read sequencing. The results showed that most cpKP isolates were categorized into clonal group 258 (CG258), in which ST11 was the dominant clone. Phylogenetic analysis revealed three major clades including the top one of Clade 3 for CG258 cpKP isolates. Additionally, carbapenemase gene analysis indicated that bla_KPC was dominant in the cpKP isolates, and most bla_KPC genes were located 2022-01-01T00:00:00Z2022 Dec2025-05-18T12:54:05.147Z2025-05-18T12:54:05.147ZS-EPMC102254883530759010.1016/j.gpb.2022.02.005