{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Freeman C"],"funding":["National Institute of Allergy and Infectious Diseases","NIAID NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["2376-2385"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10227724"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["32(9)"],"pubmed_abstract":["Up to 80% of the fatty acids in <i>Staphylococcus aureus</i> membrane lipids are branched, rather than straight-chain, fatty acids. The branched fatty acids (BCFAs) may have either an even or odd number of carbons, and the branch position may be at the penultimate carbon (<i>iso</i>) or the antepenultimate (<i>anteiso</i>) carbon of the tail. This results in two sets of isomeric fatty acid species with the same number of carbons that cannot be resolved by mass spectrometry. The isomer/isobar challenge is further complicated when the mixture of BCFAs and straight-chain fatty acids (SCFAs) are esterified into diacylated lipids such as the phosphatidylglycerol (PG) species of the <i>S. aureus</i> membrane. No conventional chromatographic method has been able to resolve diacylated lipids conta"],"journal":["Journal of the American Society for Mass Spectrometry"],"pubmed_title":["Revealing Fatty Acid Heterogeneity in Staphylococcal Lipids with Isotope Labeling and RPLC-IM-MS."],"pmcid":["PMC10227724"],"funding_grant_id":["R15GM061583","1R21AI13535","R15 GM061583","K22 AI143919","K22AI143919"],"pubmed_authors":["Gatto C","Carpenter JM","Hynds HM","Barbarek S","Freeman C","Wilkinson BJ","Hines KM","Bimpeh K","Appala K"],"additional_accession":[]},"is_claimable":false,"name":"Revealing Fatty Acid Heterogeneity in Staphylococcal Lipids with Isotope Labeling and RPLC-IM-MS.","description":"Up to 80% of the fatty acids in <i>Staphylococcus aureus</i> membrane lipids are branched, rather than straight-chain, fatty acids. The branched fatty acids (BCFAs) may have either an even or odd number of carbons, and the branch position may be at the penultimate carbon (<i>iso</i>) or the antepenultimate (<i>anteiso</i>) carbon of the tail. This results in two sets of isomeric fatty acid species with the same number of carbons that cannot be resolved by mass spectrometry. The isomer/isobar challenge is further complicated when the mixture of BCFAs and straight-chain fatty acids (SCFAs) are esterified into diacylated lipids such as the phosphatidylglycerol (PG) species of the <i>S. aureus</i> membrane. No conventional chromatographic method has been able to resolve diacylated lipids conta","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-07-08T03:10:28.724Z","creation":"2025-07-08T03:10:28.724Z"},"accession":"S-EPMC10227724","cross_references":{"pubmed":["34014662"],"doi":["10.1021/jasms.1c00092"]}}