biostudies-literatureTian CXSpecial Funds for the Cultivation of Guangdong College Students’ Scientific and Technological InnovationGuangdong Basic and Applied Basic Research FoundationSpecial Funds for the Cultivation of Guangdong College Students' Scientific and Technological InnovationModern Seed Industry Park for Whiteleg Shrimp of Guangdong ProvinceNational College Students Innovation and Entrepreneurship Training ProgramSelf-financing Project of Guangxi Agricultural Science and Technology291https://www.ebi.ac.uk/biostudies/studies/S-EPMC10230808biostudies-literatureUnknown24(1)<h4>Background</h4>Hong Kong catfish (Clarias fuscus) is an ecologically and economically important species that is widely distributed in freshwater regions of southern China. Hong Kong catfish has significant sexual growth dimorphism. The genome assembly of the Hong Kong catfish would facilitate study of the sex determination and evolution mechanism of the species.<h4>Results</h4>The first high-quality chromosome-level genome of the Hong Kong catfish was constructed. The total genome was 933.4 Mb, with 416 contigs and a contig N50 length of 8.52 Mb. Using high-throughput chromosome conformation capture (Hi-C) data, the genome assembly was divided into 28 chromosomes with a scaffold N50 length of 36.68 Mb. A total of 23,345 protein-coding genes were predicted in the genome, and 94.28% of tBMC genomicsA chromosome-level genome assembly of Hong Kong catfish (Clarias fuscus) uncovers a sex-determining region.PMC10230808pdjh2022b0239K22221Z20191232019A1515110619202210566003Duan CYTian CXZhang YLLin XHYang BLLi GLChen YZhou DYYe MHShen YJDeng SPZhu CHfalseA chromosome-level genome assembly of Hong Kong catfish (Clarias fuscus) uncovers a sex-determining region.<h4>Background</h4>Hong Kong catfish (Clarias fuscus) is an ecologically and economically important species that is widely distributed in freshwater regions of southern China. Hong Kong catfish has significant sexual growth dimorphism. The genome assembly of the Hong Kong catfish would facilitate study of the sex determination and evolution mechanism of the species.<h4>Results</h4>The first high-quality chromosome-level genome of the Hong Kong catfish was constructed. The total genome was 933.4 Mb, with 416 contigs and a contig N50 length of 8.52 Mb. Using high-throughput chromosome conformation capture (Hi-C) data, the genome assembly was divided into 28 chromosomes with a scaffold N50 length of 36.68 Mb. A total of 23,345 protein-coding genes were predicted in the genome, and 94.28% of t2023-01-01T00:00:00Z2023 May2025-06-28T03:06:18.59Z2025-04-21T21:50:26.889ZS-EPMC102308083725405510.1186/s12864-023-09394-2