biostudies-literatureRay ALAmerican Cancer SocietyNCI NIH HHSNational Institutes of HealthNIGMS NIH HHS2158-2169https://www.ebi.ac.uk/biostudies/studies/S-EPMC10239359biostudies-literatureUnknown29(11)<h4>Purpose</h4>G-CSF enhances colon cancer development. This study defines the prevalence and effects of increased G-CSF signaling in human colon cancers and investigates G-CSF inhibition as an immunotherapeutic strategy against metastatic colon cancer.<h4>Experimental design</h4>Patient samples were used to evaluate G-CSF and G-CSF receptor (G-CSFR) levels by IHC with sera used to measure G-CSF levels. Peripheral blood mononuclear cells were used to assess the rate of G-CSFR+ T cells and IFNγ responses to chronic ex vivo G-CSF. An immunocompetent mouse model of peritoneal metastasis (MC38 cells in C57Bl/6J) was used to determine the effects of G-CSF inhibition (αG-CSF) on survival and the tumor microenvironment (TME) with flow and mass cytometry.<h4>Results</h4>In human colon cancer sampClinical cancer research : an official journal of the American Association for Cancer ResearchG-CSF Is a Novel Mediator of T-Cell Suppression and an Immunotherapeutic Target for Women with Colon Cancer.PMC10239359P20GM103639-6146MRSG 15-136-01-CCEP30 CA225520P20 GM103639Fung KMLerner MRKamal MGuo SUrdaneta-Perez MGSaunders ASLang MLNofchissey RAHanson JACloyde MLewis SEReidy MARay ALMorris KTfalseG-CSF Is a Novel Mediator of T-Cell Suppression and an Immunotherapeutic Target for Women with Colon Cancer.<h4>Purpose</h4>G-CSF enhances colon cancer development. This study defines the prevalence and effects of increased G-CSF signaling in human colon cancers and investigates G-CSF inhibition as an immunotherapeutic strategy against metastatic colon cancer.<h4>Experimental design</h4>Patient samples were used to evaluate G-CSF and G-CSF receptor (G-CSFR) levels by IHC with sera used to measure G-CSF levels. Peripheral blood mononuclear cells were used to assess the rate of G-CSFR+ T cells and IFNγ responses to chronic ex vivo G-CSF. An immunocompetent mouse model of peritoneal metastasis (MC38 cells in C57Bl/6J) was used to determine the effects of G-CSF inhibition (αG-CSF) on survival and the tumor microenvironment (TME) with flow and mass cytometry.<h4>Results</h4>In human colon cancer samp2023-01-01T00:00:00Z2023 Jun2026-05-28T11:24:37.586Z2024-11-12T08:29:29.986ZS-EPMC102393593695168210.1158/1078-0432.ccr-22-391810.1158/1078-0432.CCR-22-3918