{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(9)"],"submitter":["Wang M"],"pubmed_abstract":["<b>Rationale:</b> Stem cells self-organize to form organoids that generate mini-organs that resemble the physiologically-developed ones. The mechanism by which the stem cells acquire the initial potential for generating mini-organs remains elusive. Here we used skin organoids as an example to study how mechanical force drives initial epidermal-dermal interaction which potentiates skin organoids to regenerate hair follicles. <b>Methods:</b> Live imaging analysis, single-cell RNA-sequencing analysis, and immunofluorescence were used to analyze the contractile force of dermal cells in skin organoids. Bulk RNA-sequencing analysis, calcium probe detection, and functional perturbations were used to verify that calcium signaling pathways respond to the contractile force of dermal cells. <i>In vit"],"journal":["Theranostics"],"pagination":["2930-2945"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10240816"],"repository":["biostudies-literature"],"pubmed_title":["Mechanical force drives the initial mesenchymal-epithelial interaction during skin organoid development."],"pmcid":["PMC10240816"],"pubmed_authors":["Jiang J","Li J","Lei M","Yang L","Zhang J","Zhou S","Wang D","Zhang M","Liu T","Zhou X","Liu W","Wang M","Zou Y","Qiu W","Li Z","Li T","Wu W","Liu D","Xu W"],"additional_accession":[]},"is_claimable":false,"name":"Mechanical force drives the initial mesenchymal-epithelial interaction during skin organoid development.","description":"<b>Rationale:</b> Stem cells self-organize to form organoids that generate mini-organs that resemble the physiologically-developed ones. The mechanism by which the stem cells acquire the initial potential for generating mini-organs remains elusive. Here we used skin organoids as an example to study how mechanical force drives initial epidermal-dermal interaction which potentiates skin organoids to regenerate hair follicles. <b>Methods:</b> Live imaging analysis, single-cell RNA-sequencing analysis, and immunofluorescence were used to analyze the contractile force of dermal cells in skin organoids. Bulk RNA-sequencing analysis, calcium probe detection, and functional perturbations were used to verify that calcium signaling pathways respond to the contractile force of dermal cells. <i>In vit","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-07-14T21:11:17.673Z","creation":"2026-06-24T03:12:15.216Z"},"accession":"S-EPMC10240816","cross_references":{"pubmed":["37284452"],"doi":["10.7150/thno.83217"]}}