biostudies-literatureUnknown13(9)Wang M<b>Rationale:</b> Stem cells self-organize to form organoids that generate mini-organs that resemble the physiologically-developed ones. The mechanism by which the stem cells acquire the initial potential for generating mini-organs remains elusive. Here we used skin organoids as an example to study how mechanical force drives initial epidermal-dermal interaction which potentiates skin organoids to regenerate hair follicles. <b>Methods:</b> Live imaging analysis, single-cell RNA-sequencing analysis, and immunofluorescence were used to analyze the contractile force of dermal cells in skin organoids. Bulk RNA-sequencing analysis, calcium probe detection, and functional perturbations were used to verify that calcium signaling pathways respond to the contractile force of dermal cells. <i>In vitTheranostics2930-2945https://www.ebi.ac.uk/biostudies/studies/S-EPMC10240816biostudies-literatureMechanical force drives the initial mesenchymal-epithelial interaction during skin organoid development.PMC10240816Jiang JLi JLei MYang LZhang JZhou SWang DZhang MLiu TZhou XLiu WWang MZou YQiu WLi ZLi TWu WLiu DXu WfalseMechanical force drives the initial mesenchymal-epithelial interaction during skin organoid development.<b>Rationale:</b> Stem cells self-organize to form organoids that generate mini-organs that resemble the physiologically-developed ones. The mechanism by which the stem cells acquire the initial potential for generating mini-organs remains elusive. Here we used skin organoids as an example to study how mechanical force drives initial epidermal-dermal interaction which potentiates skin organoids to regenerate hair follicles. <b>Methods:</b> Live imaging analysis, single-cell RNA-sequencing analysis, and immunofluorescence were used to analyze the contractile force of dermal cells in skin organoids. Bulk RNA-sequencing analysis, calcium probe detection, and functional perturbations were used to verify that calcium signaling pathways respond to the contractile force of dermal cells. <i>In vit2023-01-01T00:00:00Z20232026-07-14T21:11:17.673Z2026-06-24T03:12:15.216ZS-EPMC102408163728445210.7150/thno.83217