biostudies-literatureUnknown13(9)Fu Y<b>Rationale:</b> Cisplatin, a potent chemotherapeutic drug, induces side effects in normal tissues including the kidney. To reduce the side effects, repeated low-dose cisplatin (RLDC) is commonly used in clinical setting. While RLDC reduces acute nephrotoxicity to certain extents, a significant portion of patients later develop chronic kidney problems, underscoring the need for novel therapeutics to alleviate the long-term sequelae of RLDC therapy. <b>Methods:</b> <i>In vivo</i>, the role of HMGB1 was examined by testing HMGB1 neutralizing antibodies in RLDC mice. <i>In vitro</i>, the effects of HMGB1 knockdown on RLDC-induced nuclear factor-κB (NF-κB) activation and fibrotic phenotype changes were tested in proximal tubular cells. To study signal transducer and activator of transcriptionTheranostics2757-2773https://www.ebi.ac.uk/biostudies/studies/S-EPMC10240827biostudies-literatureThe STAT1/HMGB1/NF-κB pathway in chronic inflammation and kidney injury after cisplatin exposure.PMC10240827Liu ZDong ZTang CCai JChen GFu YXiang YZha JWang YYang DfalseThe STAT1/HMGB1/NF-κB pathway in chronic inflammation and kidney injury after cisplatin exposure.<b>Rationale:</b> Cisplatin, a potent chemotherapeutic drug, induces side effects in normal tissues including the kidney. To reduce the side effects, repeated low-dose cisplatin (RLDC) is commonly used in clinical setting. While RLDC reduces acute nephrotoxicity to certain extents, a significant portion of patients later develop chronic kidney problems, underscoring the need for novel therapeutics to alleviate the long-term sequelae of RLDC therapy. <b>Methods:</b> <i>In vivo</i>, the role of HMGB1 was examined by testing HMGB1 neutralizing antibodies in RLDC mice. <i>In vitro</i>, the effects of HMGB1 knockdown on RLDC-induced nuclear factor-κB (NF-κB) activation and fibrotic phenotype changes were tested in proximal tubular cells. To study signal transducer and activator of transcription2023-01-01T00:00:00Z20232026-05-29T03:28:01.781Z2025-04-06T11:13:49.751ZS-EPMC102408273728444610.7150/thno.81406