{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(1)"],"submitter":["Wahba MI"],"funding":["National Research Centre Egypt","National Research Centre"],"pubmed_abstract":["Three polysaccharide matrices (κ-Carrageenan (Carr), gellan gum, and agar) were grafted via glutaraldehyde (GA) and pea protein (PP). The grafted matrices covalently immobilized β-D-galactosidase (β-GL). Nonetheless, grafted Carr acquired the topmost amount of immobilized β-GL (iβ-GL). Thus, its grafting process was honed via Box-Behnken design and was further characterized via FTIR, EDX, and SEM. The optimal GA-PP-Carr grafting comprised processing Carr beads with 10% PP dispersion of pH 1 and 25% GA solution. The optimal GA-PP-Carr beads acquired 11.44 Ug<sup>-1</sup> iβ-GL with 45.49% immobilization efficiency. Both free and GA-PP-Carr iβ-GLs manifested their topmost activity at the selfsame temperature and pH. Nonetheless, the β-GL K<sub>m</sub> and V<sub>max</sub> values were reduced "],"journal":["Scientific reports"],"pagination":["9105"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10241775"],"repository":["biostudies-literature"],"pubmed_title":["Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers."],"pmcid":["PMC10241775"],"pubmed_authors":["Wahba MI"],"additional_accession":[]},"is_claimable":false,"name":"Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers.","description":"Three polysaccharide matrices (κ-Carrageenan (Carr), gellan gum, and agar) were grafted via glutaraldehyde (GA) and pea protein (PP). The grafted matrices covalently immobilized β-D-galactosidase (β-GL). Nonetheless, grafted Carr acquired the topmost amount of immobilized β-GL (iβ-GL). Thus, its grafting process was honed via Box-Behnken design and was further characterized via FTIR, EDX, and SEM. The optimal GA-PP-Carr grafting comprised processing Carr beads with 10% PP dispersion of pH 1 and 25% GA solution. The optimal GA-PP-Carr beads acquired 11.44 Ug<sup>-1</sup> iβ-GL with 45.49% immobilization efficiency. Both free and GA-PP-Carr iβ-GLs manifested their topmost activity at the selfsame temperature and pH. Nonetheless, the β-GL K<sub>m</sub> and V<sub>max</sub> values were reduced ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-04T14:40:30.82Z","creation":"2025-04-04T14:40:30.82Z"},"accession":"S-EPMC10241775","cross_references":{"pubmed":["37277367"],"doi":["10.1038/s41598-023-36045-z"]}}