biostudies-literatureKongsomboonvech AKHellman FoundationNIAID NIH HHSNational Institutes of Health1130965https://www.ebi.ac.uk/biostudies/studies/S-EPMC10242045biostudies-literatureUnknown13<h4>Introduction</h4><i>Toxoplasma gondii</i> induces a strong CD8 T cell response characterized by the secretion of IFNγ that promotes host survival during infection. The initiation of CD8 T cell IFNγ responses <i>in vitro</i> differs widely between clonal lineage strains of <i>T. gondii</i>, in which type I strains are low inducers, while types II and III strains are high inducers. We hypothesized this phenotype is due to a polymorphic "Regulator Of CD8 T cell Response" (ROCTR).<h4>Methods</h4>Therefore, we screened F1 progeny from genetic crosses between the clonal lineage strains to identify ROCTR. Naïve antigen-specific CD8 T cells (T57) isolated from transnuclear mice, which are specific for the endogenous and vacuolar TGD057 antigen, were measured for their ability to become activatFrontiers in cellular and infection microbiologyVariation in CD8 T cell IFNγ differentiation to strains of <i>Toxoplasma gondii</i> is characterized by small effect QTLs with contribution from ROP16.PMC10242045R01 AI137126R15 AI131027Garcia-Lopez LRosenberg ARodriguez FNjume FJensen KDCKongsomboonvech AKSouza SPfalseVariation in CD8 T cell IFNγ differentiation to strains of <i>Toxoplasma gondii</i> is characterized by small effect QTLs with contribution from ROP16.<h4>Introduction</h4><i>Toxoplasma gondii</i> induces a strong CD8 T cell response characterized by the secretion of IFNγ that promotes host survival during infection. The initiation of CD8 T cell IFNγ responses <i>in vitro</i> differs widely between clonal lineage strains of <i>T. gondii</i>, in which type I strains are low inducers, while types II and III strains are high inducers. We hypothesized this phenotype is due to a polymorphic "Regulator Of CD8 T cell Response" (ROCTR).<h4>Methods</h4>Therefore, we screened F1 progeny from genetic crosses between the clonal lineage strains to identify ROCTR. Naïve antigen-specific CD8 T cells (T57) isolated from transnuclear mice, which are specific for the endogenous and vacuolar TGD057 antigen, were measured for their ability to become activat2023-01-01T00:00:00Z20232026-04-08T13:13:58.153Z2024-11-21T09:32:57.507ZS-EPMC102420453728746610.3389/fcimb.2023.1130965