biostudies-literatureMa JNational Natural Science Foundation ProjectNatural Science Foundation Project of ChongqingJoint Medical Research Project of Chongqing Health Commission and Science and Technology Bureau252https://www.ebi.ac.uk/biostudies/studies/S-EPMC10243060biostudies-literatureUnknown23(1)<h4>Background</h4>Primary Sjögren's syndrome (pSS) is a chronic autoimmune disorder defined by xerostomia and keratoconjunctivitis sicca, and its etiology remains unknown. N6-methyladenosine (m⁶A) is the predominant posttranscriptional modification in eukaryotic mRNAs and is dynamically regulated by m⁶A regulators. Dysregulation of m⁶A modification is closely associated with several autoimmune disorders, but the role of m⁶A modification in pSS remains unknown. This study investigated the potential role of m⁶A and m⁶A-related regulators in pSS patients with dry eye.<h4>Methods</h4>This cross-sectional study included forty-eight pSS patients with dry eye and forty healthy controls (HCs). Peripheral blood mononuclear cells (PBBMC ophthalmologyIncreased METTL3 expression and m⁶A RNA methylation may contribute to the development of dry eye in primary Sjogren's syndrome.PMC10243060820709352019MSXM003cstc2019jcyj-msxmX0398Yang XFang HTan TMa JWang XZhong YZhang QfalseIncreased METTL3 expression and m⁶A RNA methylation may contribute to the development of dry eye in primary Sjogren's syndrome.<h4>Background</h4>Primary Sjögren's syndrome (pSS) is a chronic autoimmune disorder defined by xerostomia and keratoconjunctivitis sicca, and its etiology remains unknown. N6-methyladenosine (m⁶A) is the predominant posttranscriptional modification in eukaryotic mRNAs and is dynamically regulated by m⁶A regulators. Dysregulation of m⁶A modification is closely associated with several autoimmune disorders, but the role of m⁶A modification in pSS remains unknown. This study investigated the potential role of m⁶A and m⁶A-related regulators in pSS patients with dry eye.<h4>Methods</h4>This cross-sectional study included forty-eight pSS patients with dry eye and forty healthy controls (HCs). Peripheral blood mononuclear cells (PB2023-01-01T00:00:00Z2023 Jun2025-04-19T14:13:47.55Z2024-11-07T00:54:35.537ZS-EPMC102430603727771610.1186/s12886-023-02988-0