{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lewis JM"],"funding":["National Health and Medical Research Council","Australian Research Council"],"pagination":["1762-1778"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10243306"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(6)"],"pubmed_abstract":["The process of O-linked protein glycosylation is highly conserved across the <i>Burkholderia</i> genus and mediated by the oligosaccharyltransferase PglL. While our understanding of <i>Burkholderia</i> glycoproteomes has increased in recent years, little is known about how <i>Burkholderia</i> species respond to modulations in glycosylation. Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of <i>Burkholderia</i>; <i>Burkholderia cenocepacia</i> K56-2, <i>Burkholderia diffusa</i> MSMB375, <i>Burkholderia multivorans</i> ATCC17616, and <i>Burkholderia thailandensis</i> E264. Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor reca"],"journal":["Journal of proteome research"],"pubmed_title":["CRISPRi-Mediated Silencing of <i>Burkholderia</i> O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts."],"pmcid":["PMC10243306"],"funding_grant_id":["2018980","FT200100270","DP210100362"],"pubmed_authors":["Lewis JM","Scott NE"],"additional_accession":[]},"is_claimable":false,"name":"CRISPRi-Mediated Silencing of <i>Burkholderia</i> O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts.","description":"The process of O-linked protein glycosylation is highly conserved across the <i>Burkholderia</i> genus and mediated by the oligosaccharyltransferase PglL. While our understanding of <i>Burkholderia</i> glycoproteomes has increased in recent years, little is known about how <i>Burkholderia</i> species respond to modulations in glycosylation. Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of <i>Burkholderia</i>; <i>Burkholderia cenocepacia</i> K56-2, <i>Burkholderia diffusa</i> MSMB375, <i>Burkholderia multivorans</i> ATCC17616, and <i>Burkholderia thailandensis</i> E264. Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor reca","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2026-06-01T21:47:14.414Z","creation":"2025-04-04T19:12:16.996Z"},"accession":"S-EPMC10243306","cross_references":{"pubmed":["36995114"],"doi":["10.1021/acs.jproteome.2c00790"]}}