{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Soleymanjahi S"],"funding":["Lawrence Pakula MD IBD Innovation fund","NCI-NIH","NIDDK NIH HHS","NIAID NIH HHS","U.S. Department of Defense","NCI NIH HHS","National Institutes of Health","NIAID","NIDDK","American Gastroenterological Association","Crohn’s and Colitis Foundation of America"],"pagination":["e161118"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10243830"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(9)"],"pubmed_abstract":["RNA-binding protein 47 (RBM47) is required for embryonic endoderm development, but a role in adult intestine is unknown. We studied intestine-specific Rbm47-knockout mice (Rbm47-IKO) following intestinal injury and made crosses into ApcMin/+ mice to examine alterations in intestinal proliferation, response to injury, and tumorigenesis. We also interrogated human colorectal polyps and colon carcinoma tissue. Rbm47-IKO mice exhibited increased proliferation and abnormal villus morphology and cellularity, with corresponding changes in Rbm47-IKO organoids. Rbm47-IKO mice adapted to radiation injury and were protected against chemical-induced colitis, with Rbm47-IKO intestine showing upregulation of antioxidant and Wnt signaling pathways as well as stem cell and developmental genes. Furthermore, Rbm47-IKO mice were protected against colitis-associated cancer. By contrast, aged Rbm47-IKO mice developed spontaneous polyposis, and Rbm47-IKO ApcMin/+ mice manifested an increased intestinal polyp burden. RBM47 mRNA was decreased in human colorectal cancer versus paired normal tissue, along with alternative splicing of tight junction protein 1 mRNA. Public databases revealed stage-specific reduction in RBM47 expression in colorectal cancer associated independently with decreased overall survival. These findings implicate RBM47 as a cell-intrinsic modifier of intestinal growth, inflammatory, and tumorigenic pathways."],"journal":["JCI insight"],"pubmed_title":["RBM47 regulates intestinal injury and tumorigenesis by modifying proliferation, oxidative response, and inflammatory pathways."],"pmcid":["PMC10243830"],"funding_grant_id":["R01 CA246208","CA239645","DK109384","P30DK056338","K08 DK132496","DK125296,DK124274","P30 DK052574","R01 CA239645","Award No. W81XWH-20-1-630","CCF#648423","NIAID R21 AI156236","Detailed below","R01 DK105129","no number assigned","Biobank","P30-DK-52574,AITAC,PAMOC","P30 DK056338","CA230289","R21 AI156236","DK-119437,DK-128169","AGA2021-5101","DK128169","DK106382,"],"pubmed_authors":["Xie Y","Soleymanjahi S","Gazit V","Byrnes K","Brown JW","Blanc V","Alvarado DM","Rubin DC","Davidson NO","Molitor EA","Mills JC","Liu TC","Ciorba MA"],"additional_accession":[]},"is_claimable":false,"name":"RBM47 regulates intestinal injury and tumorigenesis by modifying proliferation, oxidative response, and inflammatory pathways.","description":"RNA-binding protein 47 (RBM47) is required for embryonic endoderm development, but a role in adult intestine is unknown. We studied intestine-specific Rbm47-knockout mice (Rbm47-IKO) following intestinal injury and made crosses into ApcMin/+ mice to examine alterations in intestinal proliferation, response to injury, and tumorigenesis. We also interrogated human colorectal polyps and colon carcinoma tissue. Rbm47-IKO mice exhibited increased proliferation and abnormal villus morphology and cellularity, with corresponding changes in Rbm47-IKO organoids. Rbm47-IKO mice adapted to radiation injury and were protected against chemical-induced colitis, with Rbm47-IKO intestine showing upregulation of antioxidant and Wnt signaling pathways as well as stem cell and developmental genes. Furthermore, Rbm47-IKO mice were protected against colitis-associated cancer. By contrast, aged Rbm47-IKO mice developed spontaneous polyposis, and Rbm47-IKO ApcMin/+ mice manifested an increased intestinal polyp burden. RBM47 mRNA was decreased in human colorectal cancer versus paired normal tissue, along with alternative splicing of tight junction protein 1 mRNA. Public databases revealed stage-specific reduction in RBM47 expression in colorectal cancer associated independently with decreased overall survival. These findings implicate RBM47 as a cell-intrinsic modifier of intestinal growth, inflammatory, and tumorigenic pathways.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2024-11-21T05:43:07.967Z","creation":"2024-11-21T05:43:07.967Z"},"accession":"S-EPMC10243830","cross_references":{"pubmed":["37014710"],"doi":["10.1172/jci.insight.161118"]}}