{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(3)"],"submitter":["Liu N"],"pubmed_abstract":["Although single-particle cryogenic electron microscopy (cryo-EM) has been applied extensively for elucidating many crucial biological mechanisms at the molecular level, this technique still faces critical challenges, the major one of which is to prepare the high-quality cryo-EM specimen. Aiming to achieve a more reproducible and efficient cryo-EM specimen preparation, novel supporting films including graphene-based two-dimensional materials have been explored in recent years. Here we report a robust and simple method to fabricate EM grids coated with single- or few-layer reduced graphene oxide (RGO) membrane in large batch for high-resolution cryo-EM structural determination. The RGO membrane has decreased interlayer space and enhanced electrical conductivity in comparison to regular graph"],"journal":["Biophysics reports"],"pagination":["227-238"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10244798"],"repository":["biostudies-literature"],"pubmed_title":["Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM."],"pmcid":["PMC10244798"],"pubmed_authors":["Xu K","Zhang J","Liu N","Wang J","Wang HW","Xu J","Peng H","Hu C","Yang Z","Zheng L","Zhang X","Chen Y","Gao X","Wang X","Cheng H","Lan J"],"additional_accession":[]},"is_claimable":false,"name":"Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM.","description":"Although single-particle cryogenic electron microscopy (cryo-EM) has been applied extensively for elucidating many crucial biological mechanisms at the molecular level, this technique still faces critical challenges, the major one of which is to prepare the high-quality cryo-EM specimen. Aiming to achieve a more reproducible and efficient cryo-EM specimen preparation, novel supporting films including graphene-based two-dimensional materials have been explored in recent years. Here we report a robust and simple method to fabricate EM grids coated with single- or few-layer reduced graphene oxide (RGO) membrane in large batch for high-resolution cryo-EM structural determination. The RGO membrane has decreased interlayer space and enhanced electrical conductivity in comparison to regular graph","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2025-04-21T13:39:43.044Z","creation":"2025-04-21T13:39:43.044Z"},"accession":"S-EPMC10244798","cross_references":{"pubmed":["37287490"],"doi":["10.52601/bpr.2021.210007"]}}