{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Zhou X"],"funding":["Swiss National Science Foundation","NIDA NIH HHS","NIMH NIH HHS"],"pubmed_abstract":["Nociceptin/orphanin-FQ (N/OFQ) is a recently appreciated critical opioid peptide with key regulatory functions in several central behavioral processes including motivation, stress, feeding, and sleep. The functional relevance of N/OFQ action in the mammalian brain remains unclear due to a lack of high-resolution approaches to detect this neuropeptide with appropriate spatial and temporal resolution. Here we develop and characterize NOPLight, a genetically encoded sensor that sensitively reports changes in endogenous N/OFQ release. We characterized the affinity, pharmacological profile, spectral properties, kinetics, ligand selectivity, and potential interaction with intracellular signal transducers of NOPLight <i>in vitro</i>. Its functionality was established in acute brain slices by exogeneous N/OFQ application and chemogenetic induction of endogenous N/OFQ release from PNOC neurons. <i>In vivo</i> studies with fibre photometry enabled direct recording of NOPLight binding to exogenous N/OFQ receptor ligands, as well as detection of endogenous N/OFQ release within the paranigral ventral tegmental area (pnVTA) during natural behaviors and chemogenetic activation of PNOC neurons. In summary, we show here that NOPLight can be used to detect N/OFQ opioid peptide signal dynamics in tissue and freely behaving animals."],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.05.26.542102"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10245933"],"repository":["biostudies-literature"],"pubmed_title":["Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release."],"pmcid":["PMC10245933"],"funding_grant_id":["P50 MH119467","310030","F31 DA059438","196455","T32 DA007278"],"pubmed_authors":["Suko A","Bhat MA","Achanta AS","Palmiter RD","Johnson JC","Bruning JC","Dernic J","Ravotto L","Zhou X","Assoumou K","Weber B","Stine C","Steuernagel L","Bauder CA","Pasqualini AL","Bruchas MR","Fusca D","Kloppenburg P","Prada PO","Jadhav S","Benke D","Patriarchi T","Stoeber M"],"additional_accession":[]},"is_claimable":false,"name":"Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release.","description":"Nociceptin/orphanin-FQ (N/OFQ) is a recently appreciated critical opioid peptide with key regulatory functions in several central behavioral processes including motivation, stress, feeding, and sleep. The functional relevance of N/OFQ action in the mammalian brain remains unclear due to a lack of high-resolution approaches to detect this neuropeptide with appropriate spatial and temporal resolution. Here we develop and characterize NOPLight, a genetically encoded sensor that sensitively reports changes in endogenous N/OFQ release. We characterized the affinity, pharmacological profile, spectral properties, kinetics, ligand selectivity, and potential interaction with intracellular signal transducers of NOPLight <i>in vitro</i>. Its functionality was established in acute brain slices by exogeneous N/OFQ application and chemogenetic induction of endogenous N/OFQ release from PNOC neurons. <i>In vivo</i> studies with fibre photometry enabled direct recording of NOPLight binding to exogenous N/OFQ receptor ligands, as well as detection of endogenous N/OFQ release within the paranigral ventral tegmental area (pnVTA) during natural behaviors and chemogenetic activation of PNOC neurons. In summary, we show here that NOPLight can be used to detect N/OFQ opioid peptide signal dynamics in tissue and freely behaving animals.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-04-08T14:11:50.831Z","creation":"2025-07-12T03:06:10.877Z"},"accession":"S-EPMC10245933","cross_references":{"pubmed":["37292957"],"doi":["10.1101/2023.05.26.542102"]}}