biostudies-literatureUnknownTamazian G<h4>Summary</h4>We present pygenomics, a Python package for working with genomic intervals and bioinformatic data files. The package implements interval operations, provides both API and CLI, and supports reading and writing data in widely used bioinformatic formats, including BAM, BED, GFF3 and VCF. The source code of pygenomics is provided with in-source documentation and type annotations and adheres to the functional programming paradigm. These features facilitate seamless integration of pygenomics routines into scripts and pipelines. The package is implemented in pure Python using its standard library only and contains the property-based testing framework. Comparison of pygenomics with other Python bioinformatic packages with relation to features and performance is presented. The perfoBioinformatics (Oxford, England)btad346https://www.ebi.ac.uk/biostudies/studies/S-EPMC10246576biostudies-literaturePygenomics: manipulating genomic intervals and data files in Python.PMC10246576Cherkasov NKanapin ASamsonova ATamazian GfalsePygenomics: manipulating genomic intervals and data files in Python.<h4>Summary</h4>We present pygenomics, a Python package for working with genomic intervals and bioinformatic data files. The package implements interval operations, provides both API and CLI, and supports reading and writing data in widely used bioinformatic formats, including BAM, BED, GFF3 and VCF. The source code of pygenomics is provided with in-source documentation and type annotations and adheres to the functional programming paradigm. These features facilitate seamless integration of pygenomics routines into scripts and pipelines. The package is implemented in pure Python using its standard library only and contains the property-based testing framework. Comparison of pygenomics with other Python bioinformatic packages with relation to features and performance is presented. The perfo2023-01-01T00:00:00Z2023 May2025-04-04T08:06:30.152Z2025-04-04T08:06:30.152ZS-EPMC102465763722801410.1093/bioinformatics/btad346