{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ooi V"],"funding":["Sea World Research and Rescue Foundation"],"pagination":["e0278792"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10246842"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(6)"],"pubmed_abstract":["Non-invasively collected faecal samples are an alternative source of DNA to tissue samples, that may be used in genetic studies of wildlife when direct sampling of animals is difficult. Although several faecal DNA extraction methods exist, their efficacy varies between species. Previous attempts to amplify mitochondrial DNA (mtDNA) markers from faeces of wild dugongs (Dugong dugon) have met with limited success and nuclear markers (microsatellites) have been unsuccessful. This study aimed to establish a tool for sampling both mtDNA and nuclear DNA (nDNA) from dugong faeces by modifying approaches used in studies of other large herbivores. First, a streamlined, cost-effective DNA extraction method that enabled the amplification of both mitochondrial and nuclear markers from large quantities"],"journal":["PloS one"],"pubmed_title":["A new DNA extraction method (HV-CTAB-PCI) for amplification of nuclear markers from open ocean-retrieved faeces of an herbivorous marine mammal, the dugong."],"pmcid":["PMC10246842"],"funding_grant_id":["SWR/1/2015; SWR/6/2016"],"pubmed_authors":["Ooi V","Hunter ME","Takoukam Kamla A","McMichael L","Lanyon JM"],"additional_accession":[]},"is_claimable":false,"name":"A new DNA extraction method (HV-CTAB-PCI) for amplification of nuclear markers from open ocean-retrieved faeces of an herbivorous marine mammal, the dugong.","description":"Non-invasively collected faecal samples are an alternative source of DNA to tissue samples, that may be used in genetic studies of wildlife when direct sampling of animals is difficult. Although several faecal DNA extraction methods exist, their efficacy varies between species. Previous attempts to amplify mitochondrial DNA (mtDNA) markers from faeces of wild dugongs (Dugong dugon) have met with limited success and nuclear markers (microsatellites) have been unsuccessful. This study aimed to establish a tool for sampling both mtDNA and nuclear DNA (nDNA) from dugong faeces by modifying approaches used in studies of other large herbivores. First, a streamlined, cost-effective DNA extraction method that enabled the amplification of both mitochondrial and nuclear markers from large quantities","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-05-28T13:42:21.023Z","creation":"2024-12-03T21:10:16.282Z"},"accession":"S-EPMC10246842","cross_references":{"pubmed":["37285349"],"doi":["10.1371/journal.pone.0278792"]}}