{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gvozdenov Z"],"funding":["Google","National Institutes of Health","NIGMS NIH HHS"],"pagination":["1786-1797.e5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10247422"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["83(11)"],"pubmed_abstract":["We measure transcriptional noise in yeast by analyzing chromatin structure and transcription of an 18-kb region of DNA whose sequence was randomly generated. Nucleosomes fully occupy random-sequence DNA, but nucleosome-depleted regions (NDRs) are much less frequent, and there are fewer well-positioned nucleosomes and shorter nucleosome arrays. Steady-state levels of random-sequence RNAs are comparable to yeast mRNAs, although transcription and decay rates are higher. Transcriptional initiation from random-sequence DNA occurs at numerous sites, indicating very low intrinsic specificity of the RNA Pol II machinery. In contrast, poly(A) profiles of random-sequence RNAs are roughly comparable to those of yeast mRNAs, suggesting limited evolutionary restraints on poly(A) site choice. Random-seq"],"journal":["Molecular cell"],"pubmed_title":["Functional analysis of a random-sequence chromosome reveals a high level and the molecular nature of transcriptional noise in yeast cells."],"pmcid":["PMC10247422"],"funding_grant_id":["R01 GM030186","R35 GM131801","F32 GM140555","GM131801","GM30186","1F32GM140555","R37 GM030186"],"pubmed_authors":["Barcutean Z","Gvozdenov Z","Struhl K"],"additional_accession":[]},"is_claimable":false,"name":"Functional analysis of a random-sequence chromosome reveals a high level and the molecular nature of transcriptional noise in yeast cells.","description":"We measure transcriptional noise in yeast by analyzing chromatin structure and transcription of an 18-kb region of DNA whose sequence was randomly generated. Nucleosomes fully occupy random-sequence DNA, but nucleosome-depleted regions (NDRs) are much less frequent, and there are fewer well-positioned nucleosomes and shorter nucleosome arrays. Steady-state levels of random-sequence RNAs are comparable to yeast mRNAs, although transcription and decay rates are higher. Transcriptional initiation from random-sequence DNA occurs at numerous sites, indicating very low intrinsic specificity of the RNA Pol II machinery. In contrast, poly(A) profiles of random-sequence RNAs are roughly comparable to those of yeast mRNAs, suggesting limited evolutionary restraints on poly(A) site choice. Random-seq","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2026-06-02T12:02:44.885Z","creation":"2025-06-27T03:05:23.391Z"},"accession":"S-EPMC10247422","cross_references":{"pubmed":["37137302"],"doi":["10.1016/j.molcel.2023.04.010"]}}