{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["482(6)"],"submitter":["Schraa SJ"],"funding":["Bayer"],"pubmed_abstract":["Tropomyosin receptor kinase (TRK) inhibitors have been approved for metastatic solid tumors harboring NTRK fusions, but the detection of NTRK fusions is challenging. International guidelines recommend pan-TRK immunohistochemistry (IHC) screening followed by next generation sequencing (NGS) in tumor types with low prevalence of NTRK fusions, including metastatic colorectal cancer (mCRC). RNA-based NGS is preferred, but is expensive, time-consuming, and extracting good-quality RNA from FFPE tissue is challenging. Alternatives in daily clinical practice are warranted. We assessed the diagnostic performance of RNA-NGS, FFPE-targeted locus capture (FFPE-TLC), fluorescence in situ hybridization (FISH), and the 5'/3' imbalance quantitative RT-PCR (qRT-PCR) after IHC screening in 268 patients with"],"journal":["Virchows Archiv : an international journal of pathology"],"pagination":["983-992"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10247849"],"repository":["biostudies-literature"],"pubmed_title":["Comparison of NTRK fusion detection methods in microsatellite-instability-high metastatic colorectal cancer."],"pmcid":["PMC10247849"],"pubmed_authors":["Feitsma H","Schraa SJ","Stelloo E","Fijneman RJA","Vink GR","de Leng WW","Bol GM","Lacle MM","Swennenhuis JF","Koopman M","Brosens LAA"],"additional_accession":[]},"is_claimable":false,"name":"Comparison of NTRK fusion detection methods in microsatellite-instability-high metastatic colorectal cancer.","description":"Tropomyosin receptor kinase (TRK) inhibitors have been approved for metastatic solid tumors harboring NTRK fusions, but the detection of NTRK fusions is challenging. International guidelines recommend pan-TRK immunohistochemistry (IHC) screening followed by next generation sequencing (NGS) in tumor types with low prevalence of NTRK fusions, including metastatic colorectal cancer (mCRC). RNA-based NGS is preferred, but is expensive, time-consuming, and extracting good-quality RNA from FFPE tissue is challenging. Alternatives in daily clinical practice are warranted. We assessed the diagnostic performance of RNA-NGS, FFPE-targeted locus capture (FFPE-TLC), fluorescence in situ hybridization (FISH), and the 5'/3' imbalance quantitative RT-PCR (qRT-PCR) after IHC screening in 268 patients with","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-19T08:11:10.657Z","creation":"2025-04-19T08:11:10.657Z"},"accession":"S-EPMC10247849","cross_references":{"pubmed":["37067589"],"doi":["10.1007/s00428-023-03538-1"]}}