biostudies-literatureUnknown482(6)Schraa SJBayerTropomyosin receptor kinase (TRK) inhibitors have been approved for metastatic solid tumors harboring NTRK fusions, but the detection of NTRK fusions is challenging. International guidelines recommend pan-TRK immunohistochemistry (IHC) screening followed by next generation sequencing (NGS) in tumor types with low prevalence of NTRK fusions, including metastatic colorectal cancer (mCRC). RNA-based NGS is preferred, but is expensive, time-consuming, and extracting good-quality RNA from FFPE tissue is challenging. Alternatives in daily clinical practice are warranted. We assessed the diagnostic performance of RNA-NGS, FFPE-targeted locus capture (FFPE-TLC), fluorescence in situ hybridization (FISH), and the 5'/3' imbalance quantitative RT-PCR (qRT-PCR) after IHC screening in 268 patients withVirchows Archiv : an international journal of pathology983-992https://www.ebi.ac.uk/biostudies/studies/S-EPMC10247849biostudies-literatureComparison of NTRK fusion detection methods in microsatellite-instability-high metastatic colorectal cancer.PMC10247849Feitsma HSchraa SJStelloo EFijneman RJAVink GRde Leng WWBol GMLacle MMSwennenhuis JFKoopman MBrosens LAAfalseComparison of NTRK fusion detection methods in microsatellite-instability-high metastatic colorectal cancer.Tropomyosin receptor kinase (TRK) inhibitors have been approved for metastatic solid tumors harboring NTRK fusions, but the detection of NTRK fusions is challenging. International guidelines recommend pan-TRK immunohistochemistry (IHC) screening followed by next generation sequencing (NGS) in tumor types with low prevalence of NTRK fusions, including metastatic colorectal cancer (mCRC). RNA-based NGS is preferred, but is expensive, time-consuming, and extracting good-quality RNA from FFPE tissue is challenging. Alternatives in daily clinical practice are warranted. We assessed the diagnostic performance of RNA-NGS, FFPE-targeted locus capture (FFPE-TLC), fluorescence in situ hybridization (FISH), and the 5'/3' imbalance quantitative RT-PCR (qRT-PCR) after IHC screening in 268 patients with2023-01-01T00:00:00Z2023 Jun2025-04-19T08:11:10.657Z2025-04-19T08:11:10.657ZS-EPMC102478493706758910.1007/s00428-023-03538-1